Rabbit anti-Human Neurofibromin/NF1 Polyclonal Antibody | anti-NF1 antibody

Anti-Neurofibromin/NF1 Antibody

Cat. Num. AAA1752908

• Gene Names: NF1; WSS; NFNS; VRNF
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Neurofibromin/NF1; Polyclonal Antibody; Anti-Neurofibromin/NF1 Antibody; Neurofibromin; Neurofibromatosis-related protein NF-1; Neurofibromin truncated; NF1; anti-NF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 2818

• Applicable Applications for anti-NF1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human); IHC-P (Embedded Section): 0.5-1ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
• Immunogen: E Coli-derived human Neurofibromin/NF1 recombinant protein (Position: R160-Q270).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Western Blot (WB)

Related Product Information for anti-NF1 antibody

Description: Rabbit IgG polyclonal antibody for Neurofibromin/NF1 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human.

Background: Neurofibromin 1 (NF1) is a gene in humans that is located on chromosome 17. This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.

References

1. Scheffzek K, Welti S (2012). "Neurofibromin: Protein Domains and Functional Characteristics". In Upadhyaya M, Cooper D (eds.). Neurofibromatosis Type 1. Berlin, Heidelberg: Springer. pp. 305-326.
2. Trovó-Marqui AB, Tajara EH (July 2006). "Neurofibromin: a general outlook". Clinical Genetics. 70 (1): 1-13.
3. Peltonen S, Pöyhönen M (2012). "Clinical Diagnosis and Atypical Forms of NF1". In Upadhyaya M, Cooper D (eds.). Neurofibromatosis Type 1. Berlin, Heidelberg: Springer. pp. 17-30.

NCBI and Uniprot Product Information

• NCBI GI #: 4557793
• NCBI GeneID: 4763
• NCBI Accession #: NP_000258.1
• NCBI GenBank Nucleotide #: NP_000258.1
• UniProt Accession #: P21359
• NCBI Official Full Name: neurofibromin isoform 2
• NCBI Official Synonym Full Names: neurofibromin 1
• NCBI Official Symbol: NF1
• NCBI Official Synonym Symbols: WSS; NFNS; VRNF
• NCBI Protein Information: neurofibromin
• UniProt Protein Name: Neurofibromin
• Protein Family: Neurofibromin
• UniProt Gene Name: NF1
• UniProt Entry Name: NF1_HUMAN

NCBI Description

This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NF1: a Ras-GAP, highly expressed in developing neural cells. Possesses tumor suppressor activity, presumably by virtue of its GTPase activating domain. Neurofibromin is phosphorylated in response to EGF in CNS cells and cell lines. Defects in NF1 are the cause of type 1 neurofibromatosis (NF1), Watson syndrome, and familial spinal neurofibromatosis. NF1 is one of the most frequent autosomal dominant diseases. Four alternatively spliced isoforms have been described.

Protein type: Tumor suppressor; GAPs, Ras; GAPs; Nucleolus; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: membrane; axon; dendrite; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; phosphatidylethanolamine binding; phosphatidylcholine binding

Biological Process: negative regulation of Rac protein signal transduction; neural tube development; negative regulation of MAP kinase activity; Schwann cell development; extracellular matrix organization and biogenesis; collagen fibril organization; positive regulation of apoptosis; heart development; negative regulation of transcription factor import into nucleus; negative regulation of neuroblast proliferation; regulation of glial cell differentiation; positive regulation of adenylate cyclase activity; negative regulation of neurotransmitter secretion; skeletal muscle development; adrenal gland development; forebrain morphogenesis; negative regulation of cell-matrix adhesion; regulation of synaptic transmission, GABAergic; myelination in the peripheral nervous system; camera-type eye morphogenesis; induction of apoptosis via death domain receptors; negative regulation of fibroblast proliferation; negative regulation of endothelial cell proliferation; negative regulation of protein kinase activity; positive regulation of endothelial cell proliferation; cerebral cortex development; actin cytoskeleton organization and biogenesis; forebrain astrocyte development; metanephros development; regulation of long-term neuronal synaptic plasticity; negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; wound healing; regulation of cell-matrix adhesion; regulation of blood vessel endothelial cell migration; smooth muscle development; sympathetic nervous system development; positive regulation of neuron apoptosis; visual learning; negative regulation of Ras protein signal transduction; cell communication; negative regulation of cell migration; regulation of bone resorption; negative regulation of MAPKKK cascade; MAPKKK cascade; liver development; regulation of angiogenesis; peripheral nervous system development; osteoblast differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of angiogenesis; pigmentation; spinal cord development; negative regulation of astrocyte differentiation; Ras protein signal transduction; response to hypoxia; artery morphogenesis; brain development; cognition

Disease: Neurofibromatosis, Familial Spinal; Watson Syndrome; Juvenile Myelomonocytic Leukemia; Neurofibromatosis, Type I; Neurofibromatosis-noonan Syndrome