Rabbit anti-Human NEURL Polyclonal Antibody | anti-NEURL antibody

NEURL, ID (NEURL, NEURL1, NEURL1A, RNF67, Neuralized-like protein 1A, RING finger protein 67) (AP)

Cat. Num. AAA6324784

• Gene Names: NEURL; neu; RNF67; neu-1; NEURL1; bA416N2.1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NEURL; Polyclonal Antibody; ID (NEURL; NEURL1; NEURL1A; RNF67; Neuralized-like protein 1A; RING finger protein 67) (AP); RING finger protein 67; anti-NEURL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NEURL antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NEURL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165~194 amino acids from the Center region of human NEURL.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NEURL Antibody (Center) in Y79 cell line lysates (35ug/lane). NEURL (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with NEURL Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-NEURL antibody

NEURL is involved in the determination of cell fate in the neurogenic region of the embryo and plays a role in the determination of cell fate in the central nervous system. NEURL may act as a tumor suppressor whose inactivation could be associated with malignant progression of astrocytic tumors.

Product Categories/Family for anti-NEURL antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Kile, B.T., et.al., Trends Biochem. Sci. 27 (5), 235-241 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 223278368
• NCBI GeneID: 9148
• NCBI Accession #: NP_004201.3
• NCBI GenBank Nucleotide #: NM_004210.4
• UniProt Accession #: O76050; Q5TDR2; Q5TDR3; Q8TAN0; Q9H463
• Molecular Weight: 61,860 Da
• NCBI Official Full Name: neuralized-like protein 1A
• NCBI Official Synonym Full Names: neuralized homolog (Drosophila)
• NCBI Official Symbol: NEURL
• NCBI Official Synonym Symbols: neu; RNF67; neu-1; NEURL1; bA416N2.1
• NCBI Protein Information: neuralized-like protein 1A; h-neuralized 1; RING finger protein 67
• UniProt Protein Name: Neuralized-like protein 1A
• UniProt Gene Name: NEURL
• UniProt Synonym Gene Names: NEURL1; NEURL1A; RNF67; h-neu
• UniProt Entry Name: NEU1A_HUMAN

Uniprot Description

NEURL1: May function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1, thereby regulating the Notch pathway. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Cell development/differentiation; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 10q25.1

Cellular Component: postsynaptic membrane; perinuclear region of cytoplasm; postsynaptic density; dendritic spine; plasma membrane; perikaryon; cell junction

Molecular Function: translation factor activity, non-nucleic acid binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: lactation; positive regulation of filopodium formation; protein monoubiquitination; nervous system development; Notch signaling pathway; positive regulation of apoptosis; positive regulation of long-term neuronal synaptic plasticity; regulation of translation; negative regulation of cell proliferation; sperm motility; positive regulation of epidermal growth factor receptor activity; sperm axoneme assembly; negative regulation of Notch signaling pathway