GRK6 Antibody | anti-GRK6 antibody

GRK6 Antibody

Cat. Num. AAA853825

• Gene Names: GRK6; GPRK6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: GRK6; GRK6 Antibody; EC 2.7.11.16; G-protein-coupled receptor kinase 6; GPRK6; kinase GRK6; anti-GRK6 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: GRK6 antibody detects endogenous levels of total GRK6 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 560

• Applicable Applications for anti-GRK6 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from N-terminal of human GRK6.
• Preparation and Storage: Store at -20°C for 1 year.

Testing Data

Western Blot (WB)

(Peptide-+ Western blot analysis of extracts from Jurkat cells, COLO205 cells and HUVEC cells, using GRK6 antibody. Immunofluorescence analysis of HeLa cells, using GRK6 antibody.)

Product Categories/Family for anti-GRK6 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Benovic J.L., J. Biol. Chem. 268:19521-19527(1993). Haribabu B., Proc. Natl. Acad. Sci. U.S.A. 90:9398-9402(1993). Stoffel R.H., J. Biol. Chem. 269:27791-27794(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 51896037
• NCBI GeneID: 2870
• NCBI Accession #: NP_001004105.1
• NCBI GenBank Nucleotide #: NM_001004105.2
• UniProt Accession #: P43250; O60541; Q13652
• Molecular Weight: 64,294 Da
• NCBI Official Full Name: G protein-coupled receptor kinase 6 isoform C
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 6
• NCBI Official Symbol: GRK6
• NCBI Official Synonym Symbols: GPRK6
• NCBI Protein Information: G protein-coupled receptor kinase 6
• UniProt Protein Name: G protein-coupled receptor kinase 6
• Protein Family: G protein-coupled receptor kinase
• UniProt Gene Name: GRK6
• UniProt Synonym Gene Names: GPRK6

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).