Rabbit ErbB2, p185 Polyclonal Antibody | anti-ERBB2 antibody

ErbB2, p185 (c-erbB2, HER2, neu Oncoprotein)

Cat. Num. AAA624467

• Gene Names: ERBB2; HER-2
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ErbB2; p185; Polyclonal Antibody; p185 (c-erbB2; HER2; neu Oncoprotein); Anti -ErbB2; anti-ERBB2 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human c-erbB-2 (185 kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4. Species Crossreactivity: monkey rat and mouse.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.

• Applicable Applications for anti-ERBB2 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 1:500 detects c-erbB-2 on 10ug of HEK293 lysates.; Immunohistochemistry: Reported to detect c-erbB-2 in frozen and formalin/paraffin sections of rat breast.; Immunoprecipitation: 4ug immunoprecipitated c-erbB-2 from 600ug of MCF-7 RIPA lysate and previously in A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysates.; Optimal dilution determined by the researcher.
• Immunogen: A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243- 1255 from the human c-erbB-2/HER-2/neu oncoprotein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MCF-7 cell lysate using MBS624467 at 1ug/ml.)

Related Product Information for anti-ERBB2 antibody

The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.

Product Categories/Family for anti-ERBB2 antibody

Antibodies; Abs to Oncoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 2575867
• NCBI GeneID: 403883
• UniProt Accession #: O18735
• Molecular Weight: 137,991 Da
• NCBI Official Full Name: erbB-2
• NCBI Official Symbol: ERBB2
• NCBI Official Synonym Symbols: HER-2
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2; c-erbB-2; p185erbB2; proto-oncogene c-ErbB-2
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB2
• UniProt Entry Name: ERBB2_CANFA

Uniprot Description

Function: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. In the nucleus, binds to the 5'-TCAAATTC-3' sequence in the MT-CO2 promoter and activates its transcription. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Homodimer. Heterodimer with ERBB3. Heterodimer with each of the other ERBB receptors

Potential. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1200. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1252) with MEMO1. Interacts with MUC1. Interacts (when phosphorylated on Tyr-1138) with GRB7 (via SH2 domain). Interacts (when phosphorylated on Tyr-1252) with ERBB2IP

Subcellular location: Cell membrane; Single-pass type I membrane protein

By similarity. Cytoplasm › perinuclear region

By similarity. Nucleus

Post-translational modification: Ligand-binding increases phosphorylation on tyrosine residues

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.Contains 1 protein kinase domain.