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Western Blot (WB) (FFAR1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FFAR1 antibody detected the FFAR1 protein (arrow).)

Rabbit anti-Human FFAR1 Polyclonal Antibody | anti-FFAR1 antibody

FFAR1, CT (FFAR1, GPR40, Free fatty acid receptor 1, G-protein coupled receptor 40)

Gene Names
FFAR1; FFA1R; GPR40; GPCR40
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
FFAR1; Polyclonal Antibody; CT (FFAR1; GPR40; Free fatty acid receptor 1; G-protein coupled receptor 40); Anti -FFAR1; anti-FFAR1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-FFAR1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
FFAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-300 amino acids from the C-terminal region of human FFAR1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FFAR1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FFAR1 antibody detected the FFAR1 protein (arrow).)

Western Blot (WB) (FFAR1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FFAR1 antibody detected the FFAR1 protein (arrow).)
Related Product Information for anti-FFAR1 antibody
This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes.
Product Categories/Family for anti-FFAR1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,457 Da
NCBI Official Full Name
free fatty acid receptor 1
NCBI Official Synonym Full Names
free fatty acid receptor 1
NCBI Official Symbol
FFAR1
NCBI Official Synonym Symbols
FFA1R; GPR40; GPCR40
NCBI Protein Information
free fatty acid receptor 1; G protein-coupled receptor 40; G-protein coupled receptor 40
UniProt Protein Name
Free fatty acid receptor 1
Protein Family
UniProt Gene Name
FFAR1
UniProt Synonym Gene Names
GPR40
UniProt Entry Name
FFAR1_HUMAN

NCBI Description

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes. [provided by RefSeq, Apr 2009]

Uniprot Description

FFAR1: Receptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G- proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; guanyl-nucleotide exchange factor activity; lipid binding; bioactive lipid receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; insulin secretion; energy reserve metabolic process; glucose homeostasis; positive regulation of calcium ion transport; regulation of insulin secretion; positive regulation of GTPase activity

Research Articles on FFAR1

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Product Notes

The FFAR1 ffar1 (Catalog #AAA6010304) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FFAR1, CT (FFAR1, GPR40, Free fatty acid receptor 1, G-protein coupled receptor 40) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FFAR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the FFAR1 ffar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FFAR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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