Western Blot (WB)
(Western Blot (WB) analysis of NEK5 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:C0S-7 whole cell lysate (40ug))
Rabbit anti-Human NEK5 Polyclonal Antibody | anti-NEK5 antibody
NEK5 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
NEK5; Polyclonal Antibody; NEK5 Polyclonal Antibody; Serine/threonine-protein kinase Nek5; Never in mitosis A-related kinase 5; NimA-related protein kinase 5; anti-NEK5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
NEK5 polyclonal antibody detects endogenous levels of NEK5 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
708
Applicable Applications for anti-NEK5 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 150-200 of Human NEK5.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of NEK5 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:C0S-7 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of NEK5 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:C0S-7 whole cell lysate (40ug))
Related Product Information for anti-NEK5 antibody
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. Nek5 (NimA-related protein kinase 5) is a 708 amino acid protein that is related to NIMA, a protein that was originally discovered in Aspergillus nidulans and is necessary for entry into mitosis. One of several members of the Ser/Thr protein kinase super family, Nek5 contains one protein kinase domain through which it catalyzes the ATP-dependent phosphorylation of target proteins. Like NIMA, Nek5 may be involved in mitotic regulation and cell cycle control.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 81kDa
NCBI Official Full Name
serine/threonine-protein kinase Nek5 isoform 1
UniProt Protein Name
Serine/threonine-protein kinase Nek5
Protein Family
UniProt Gene Name
NEK5
UniProt Synonym Gene Names
NimA-related protein kinase 5
UniProt Entry Name
NEK5_HUMAN
Uniprot Description
NEK5: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Protein type: Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; NEK family
Chromosomal Location of Human Ortholog: 13q14.3
Cellular Component: nucleus
Molecular Function: protein serine/threonine kinase activity; metal ion binding; ATP binding
Biological Process: positive regulation of striated muscle cell differentiation; protein amino acid phosphorylation
Similar Products
Product Notes
The NEK5 nek5 (Catalog #AAA3007618) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEK5 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEK5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the NEK5 nek5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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