Western Blot (WB)
(Western blot analysis of anti-KIS Pab in mouse heart tissue lysate. KIS (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human, Mouse KIST Polyclonal Antibody | anti-KIST antibody
KIST, NT (UHMK1, KIS, KIST, Serine/threonine-protein kinase Kist, Kinase interacting with stathmin, U2AF homology motif kinase 1) (APC)
Gene Names
UHMK1; KIS; KIST; P-CIP2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
KIST; Polyclonal Antibody; NT (UHMK1; KIS; Serine/threonine-protein kinase Kist; Kinase interacting with stathmin; U2AF homology motif kinase 1) (APC); UHMK1; U2AF homology motif kinase 1; anti-KIST antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-KIST antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
KIS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human KIS.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-KIS Pab in mouse heart tissue lysate. KIS (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-KIS Pab in mouse heart tissue lysate. KIS (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(KIS Antibody (C6) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KIS antibody detected the KIS protein (arrow).)
Western Blot (WB)
(KIS Antibody (C6) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KIS antibody detected the KIS protein (arrow).)
Related Product Information for anti-KIST antibody
Upon serum stimulation, KIS, a member of the Ser/Thr protein kinase family, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. This protein, which contains 1 RRM (RNA recognition motif)domain, may be involved in trafficking and/or processing of RNA. KIS is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes.
Product Categories/Family for anti-KIST antibody
References
Bieche, I., et al., Brain Res. Mol. Brain Res. 114(1):55-64 (2003). Boehm, M., et al., EMBO J. 21(13):3390-3401 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,044 Da
NCBI Official Full Name
serine/threonine-protein kinase Kist isoform 2
NCBI Official Synonym Full Names
U2AF homology motif (UHM) kinase 1
NCBI Official Symbol
UHMK1
NCBI Official Synonym Symbols
KIS; KIST; P-CIP2
NCBI Protein Information
serine/threonine-protein kinase Kist; KIS protein kinase; PAM COOH-terminal interactor protein 2; kinase interacting with leukemia-associated gene (stathmin)
UniProt Protein Name
Serine/threonine-protein kinase Kist
UniProt Gene Name
UHMK1
UniProt Synonym Gene Names
KIS; KIST; P-CIP2
UniProt Entry Name
UHMK1_HUMAN
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Product Notes
The KIST uhmk1 (Catalog #AAA6313469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KIST, NT (UHMK1, KIS, KIST, Serine/threonine-protein kinase Kist, Kinase interacting with stathmin, U2AF homology motif kinase 1) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KIST can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KIST uhmk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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