Cadherin T Active Protein

Cadherin T, Recombinant, Human (Truncated Cadherin, Cadherin 13, Heart-Cadherin)

Cat. Num. AAA637241

• Purity: Highly Purified; 90%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Cadherin T; Recombinant; Human (Truncated Cadherin; Cadherin 13; Heart-Cadherin); Cadherin T active protein

• Purity/Purification: Highly Purified; 90%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized from a 0.2um filtered solution in MES and NaCl. Reconstitute at 200ug/ml in PBS.

• Activity: Measured by the ability of the immobilized protein to inhibit the adhesion of human umbilical vein endothelial cells (HUVEC). When 5x10e4 cells/well are added to rhCadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2 hours at 37 degree C. The ED50 for this effect is typically 2-8ug/ml.
• Endotoxin Level: 1.0 EU per 1ug of the protein by the LAL method.
• Preparation and Storage: -20 degree C

Testing Data

(Cadherin-13 Inhibits Adhesion of Human Umbilical Vein Endothelial Cells. Human umbilical vein endothelial cells (HUVEC) were added to plates coated with the indicated concentrations of recombinant human Cadherin-13. After two hours, the plates were washed and the number of adherent cells was determined.)

Related Product Information for Cadherin T active protein

Cadherin­13, also known as T­cadherin and H­cadherin, is a 105 kDa member of the cadherin family of transmembrane glycoproteins that mediate calcium­dependent intercellular adhesion (1). However, Cadherin­13 is an atypical member, lacking transmembrane and cytosolic domains and containing a GPI moiety that anchors Cadherin­13 to the plasma membrane (1 ­ 2). Human Cadherin­13 is synthesized as a 713 amino acid (aa) precursor that contains a 22 aa signal sequence, a 116 aa propeptide, a 555 aa mature chain, and a second propeptide of 20 aa that is removed in the mature form to reveal the GPI anchor (SwissProt # P55290). The mature form contains five cadherin domains and eight potential sites for N­linked glycosylation. Mature human Cadherin­13 shares 96% aa identity with mature mouse Cadherin­13. Cadherin­13 is expressed in various tissues. It is highly expressed in the heart, and in the CNS, Cadherin­13 is expressed in the cerebral cortex, medulla, hippocampus, amygdala, thalamus, and substantia nigra (2). There are higher levels of Cadherin­13 in the adult brain than in developing brain (2). Cadherin­13 is also expressed in skin in the basal layer of the epidermis, lung, liver, kidney, and blood vessels (2). The structural characteristics of Cadherin­13 predict that it is unlikely to function as a true adhesion molecule in vivo (2). It is suggested that it may act rather as a signaling receptor participating in recognition of the environment and regulation of cell motility, proliferation, and phenotype (2). Cellular expression levels of Cadherin­13 in various tissues often correlate, negatively or positively, with the proliferative potential of the cells (2). Cadherin­13 may also act as a suppressor of tumor cell growth (2). This potential role for Cadherin­13 was emphasized by localization of Cadherin­13 gene to chromosome 16q24, a region exhibiting loss of heterozygosity in many solid tumors (2). Allelic loss of chromosome bands 16q24.1­q24.2 and reduced expression of Cadherin­13, as well as hypermethylation of the remaining allele have been detected in a considerable number of human cancers (2).

Product Categories/Family for Cadherin T active protein

Molecular Biology; MB-Cadherin

NCBI and Uniprot Product Information

• NCBI GI #: 386422722
• NCBI Official Full Name: Cadherin