Rabbit NEIL3 Polyclonal Antibody | anti-NEIL3 antibody

NEIL3 Polyclonal Antibody

Cat. Num. AAA3007722

• Gene Names: NEIL3; FGP2; FPG2; NEI3; ZGRF3; hFPG2; hNEI3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NEIL3; Polyclonal Antibody; NEIL3 Polyclonal Antibody; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK; PTK2; FAK; FAK1; anti-NEIL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NEIL3 polyclonal antibody detects endogenous levels of NEIL3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 605

• Applicable Applications for anti-NEIL3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human PTK2 around the phosphorylation site of Serine 843
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NEIL3 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:The Thymus tissue lysate of Rat (40ug) Lane4:The Testis tissue lysate of Mouse (40ug))

Related Product Information for anti-NEIL3 antibody

NEIL1, NEIL2 and NEIL3, also known as endonuclease VIII-like 1, 2 and 3 or DNA-(apurinic or apyrimidinic site) lyase NEIL 1, 2 and 3, are nuclear proteins involved in the repair of DNA damaged by oxidation. The NEIL proteins belong to the FPG family. They act as DNA glycosylases that can recognize and remove damaged bases, leaving an abasic site. NEIL3, however, lacks the proline residue at the N-terminus which acts as the active site residue found in NEIL1 and NEIL2. Thus, reports of NEIL3 DNA glycosylase activity are contradictory. NEIL3 localizes to the nucleus and only demonstrates expression in thymus and testis tissues. The deduced 605 amino acid NEIL3 protein contains one FPG-type zinc finger and one RanBP2-type zinc finger.

NCBI and Uniprot Product Information

• NCBI GI #: 157388969
• NCBI GeneID: 55247
• NCBI Accession #: NP_060718.2
• NCBI GenBank Nucleotide #: NP_060718.2
• UniProt Accession #: Q8TAT5
• Molecular Weight: ~ 67kDa
• NCBI Official Full Name: endonuclease 8-like 3
• NCBI Official Synonym Full Names: nei like DNA glycosylase 3
• NCBI Official Symbol: NEIL3
• NCBI Official Synonym Symbols: FGP2; FPG2; NEI3; ZGRF3; hFPG2; hNEI3
• NCBI Protein Information: endonuclease 8-like 3
• UniProt Protein Name: Endonuclease 8-like 3
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL3
• UniProt Entry Name: NEIL3_HUMAN

NCBI Description

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]

Uniprot Description

NEIL3: Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single- stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected. Belongs to the FPG family.

Protein type: EC 4.2.99.18; DNA-binding

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: nucleus

Molecular Function: DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; double-stranded DNA binding; DNA N-glycosylase activity; damaged DNA binding; bubble DNA binding; single-stranded DNA binding

Biological Process: nucleotide-excision repair; base-excision repair; DNA catabolic process, endonucleolytic