Rabbit Neddylin Polyclonal Antibody | anti-D21 antibody

Neddylin (D21) Polyclonal Antibody

Cat. Num. AAA3001121

• Gene Names: NEDD8; NEDD-8
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Neddylin; Polyclonal Antibody; Neddylin (D21) Polyclonal Antibody; NEDD8; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8; anti-D21 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Neddylin (D21) polyclonal antibody detects endogenous levels of Neddylin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 81

• Applicable Applications for anti-D21 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Neddylin.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Neddylin (D21) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.)

Related Product Information for anti-D21 antibody

NEDD8 is a ubiquitin (Ub)-like molecule that shares 80% homology with ubiquitin, a protein known to modify and target various proteins for proteolytic degradation. NEDD8 and the corresponding yeast homolog RUB1 are activated by the E1 ubiquitin activating enzyme UBA2, which forms isopeptide linkages between thio esters. Similar to the Ub-mediated proteolytic pathway, NEDD8 is covalently coupled to an E3 Ub ligase by the E2 conjugating enzyme, UBC12. NEDD8 preferentially associates with the CUL-2 protein in the E3 ligase complex CBCVHL, which consists of cullin proteins associating with elongin B/C and the tumor suppressor F-box protein von Hippel Lindau (VHL). NEDD8 is predominantly localized to the nucleus and is highly expressed in adult heart and skeletal muscle. In vitro studies indicate that NEDD8 tetramers are also able to bind to the 26S proteasome, and that they assemble into complexes with conserved Ub-like moieties, suggesting that NEDD8 may regulate proteolysis of intracellular proteins similar to other Ub-mediated pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 5453760
• NCBI GeneID: 4738
• NCBI Accession #: NP_006147.1
• NCBI GenBank Nucleotide #: NP_006147.1
• UniProt Accession #: Q15843
• Molecular Weight: ~ 9kDa
• NCBI Official Full Name: NEDD8
• NCBI Official Synonym Full Names: NEDD8 ubiquitin like modifier
• NCBI Official Symbol: NEDD8
• NCBI Official Synonym Symbols: NEDD-8
• NCBI Protein Information: NEDD8
• UniProt Protein Name: NEDD8
• UniProt Gene Name: NEDD8
• UniProt Synonym Gene Names: NEDD-8
• UniProt Entry Name: NEDD8_HUMAN

Uniprot Description

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; anatomical structure morphogenesis; protein localization; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance