Testing Data
(All lanes: Anti ALPL (MBS176908) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: HT1080 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 57KDObserved bind size: 57KD )
Rabbit anti-Human Alkaline Phosphatase Polyclonal Antibody | anti-ALPL antibody
Anti-Alkaline Phosphatase antibody
Testing Data
(All lanes: Anti ALPL (MBS176908) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: HT1080 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 57KDObserved bind size: 57KD )
Testing Data
(All lanes: Anti ALPL (MBS176908) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: HT1080 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 57KDObserved bind size: 57KD )
Background: Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
NCBI and Uniprot Product Information
NCBI Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
Uniprot Description
ALPL: This isozyme may play a role in skeletal mineralization. Defects in ALPL are a cause of hypophosphatasia (HOPS). HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC). Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI). Belongs to the alkaline phosphatase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.1; Cofactor and Vitamin Metabolism - folate biosynthesis; Phosphatase (non-protein); Motility/polarity/chemotaxis; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 1p36.12
Cellular Component: extracellular matrix; extracellular space; membrane; plasma membrane; integral to membrane
Molecular Function: pyrophosphatase activity; protein binding; alkaline phosphatase activity; metal ion binding
Biological Process: response to antibiotic; osteoblast differentiation; response to vitamin D; dephosphorylation; response to glucocorticoid stimulus; reproductive developmental process; response to lipopolysaccharide; skeletal development; endochondral ossification
Disease: Hypophosphatasia, Infantile; Hypophosphatasia, Adult; Hypophosphatasia, Childhood
Research Articles on ALPL
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Product Notes
The ALPL alpl (Catalog #AAA176908) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alkaline Phosphatase antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alkaline Phosphatase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ALPL alpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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