Western Blot (WB)
(Western blot analysis of NEDD8 expression in BJAB (A), K562 (B), HeLa (C) whole cell lysates.)
Rabbit NEDD8 Polyclonal Antibody | anti-NEDD8 antibody
Anti-NEDD8 Antibody
Western Blot (WB)
(Western blot analysis of NEDD8 expression in BJAB (A), K562 (B), HeLa (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of NEDD8 expression in BJAB (A), K562 (B), HeLa (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
Uniprot Description
NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.
Protein type: Ubiquitin-like modifier
Chromosomal Location of Human Ortholog: 14q12
Cellular Component: nucleus; cytosol
Molecular Function: protein binding; ubiquitin protein ligase binding
Biological Process: ubiquitin-dependent protein catabolic process; regulation of transcription from RNA polymerase II promoter; protein localization; anatomical structure morphogenesis; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance
Research Articles on NEDD8
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Product Notes
The NEDD8 nedd8 (Catalog #AAA820582) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NEDD8 Antibody reacts with Human, Mouse, Rat, Bovine, Dog, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the NEDD8 nedd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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