Rabbit NEDD8 Polyclonal Antibody | anti-NEDD8 antibody

Rabbit Polyclonal (IgG) to Human NEDD8

Cat. Num. AAA247184

• Gene Names: NEDD8; NEDD-8
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NEDD8; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human NEDD8; Anti-NEDD8 Antibody (aa10-59) IHC-plus; NEDD-8; Neddylin; Ubiquitin-like protein Nedd8; Human NEDD8; anti-NEDD8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NEDD8 Antibody detects endogenous levels of total NEDD8 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 81

• Applicable Applications for anti-NEDD8 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IHC-P (1:100), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human NEDD8 (aa10-59).
• Immunogen: NEDD8 antibody was raised against synthetic peptide from human NEDD8 (aa10-59).
• Antigen Modification: aa10-59
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-NEDD8 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(Western blot of extracts from Jurkat cells, using NEDD8 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 5453760
• NCBI GeneID: 4738
• NCBI Accession #: NP_006147.1
• NCBI GenBank Nucleotide #: NM_006156.2
• UniProt Accession #: Q15843; Q3SXN8; Q6LES6
• Molecular Weight: 9,072 Da
• NCBI Official Full Name: NEDD8
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 8
• NCBI Official Symbol: NEDD8
• NCBI Official Synonym Symbols: NEDD-8
• NCBI Protein Information: NEDD8; neddylin; ubiquitin-like protein Nedd8
• UniProt Protein Name: NEDD8
• Protein Family: NEDD8
• UniProt Gene Name: NEDD8
• UniProt Synonym Gene Names: NEDD-8
• UniProt Entry Name: NEDD8_HUMAN

Uniprot Description

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: nucleus; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: ubiquitin-dependent protein catabolic process; regulation of transcription from RNA polymerase II promoter; protein neddylation; protein localization; anatomical structure morphogenesis; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance