Rabbit NDUFA2 Polyclonal Antibody | anti-NDUFA2 antibody

NDUFA2 Antibody (C-Term)

Cat. Num. AAA9231300

• Gene Names: NDUFA2; B8; CD14; CIB8
• Reactivity: Human; Predicted: Bovine
• Applications: Western Blot, ELISA
• Purity: Purified through a protein A column, followed by peptide affinity purification.
• Synonyms: NDUFA2; Polyclonal Antibody; NDUFA2 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; Complex I-B8; CI-B8; NADH-ubiquinone oxidoreductase B8 subunit; anti-NDUFA2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Bovine
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This NDUFA2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 62-96 amino acids from the human region of human NDUFA2.
• Purity/Purification: Purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 76

• Applicable Applications for anti-NDUFA2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks.; For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-NDUFA2 Antibody (C-Term) at 1:2000 dilution + Human heart lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NDUFA2 antibody

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Product Categories/Family for anti-NDUFA2 antibody

Primary Antibodies; Cancer; Metabolism; Signal Transduction

References

Ton C.,et al.Biochem. Biophys. Res. Commun. 241:589-594(1997). Iida A.,et al. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases. Peng Y.,et al. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases. Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000). Ebert L.,et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 297206774
• NCBI GeneID: 4695
• NCBI Accession #: NP_001171941.1
• NCBI GenBank Nucleotide #: NM_001185012.1
• UniProt Accession #: O43678; Q6IAY8; D6RJD6
• Molecular Weight: 10922
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase subunit A2
• NCBI Official Symbol: NDUFA2
• NCBI Official Synonym Symbols: B8; CD14; CIB8
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
• UniProt Gene Name: NDUFA2
• UniProt Synonym Gene Names: CI-B8

NCBI Description

The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.