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Testing Data (Gel: 8%SDS-PAGE Lysates (from left to right): Hela and A549 cell Amount of lysate: 40ug per lane Primary antibody: 1/500 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 minutes)

Rabbit anti-Human NDUFA10 Polyclonal Antibody | anti-NDUFA10 antibody

NDUFA10 Antibody

Gene Names
NDUFA10; CI-42k; CI-42KD
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
NDUFA10; Polyclonal Antibody; NDUFA10 Antibody; CI-42k; CI-42KD; anti-NDUFA10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total NDUFA10 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
2 mg/ml (varies by lot)
Sequence Length
355
Applicable Applications for anti-NDUFA10 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:50-1:200
Immunogen Type
Recombinant Protein
Immunogen Description
Fusion protein corresponding to a region derived from internal residues of human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
Target Name
NDUFA10
Preparation and Storage
Store at -20 degree C

Testing Data

(Gel: 8%SDS-PAGE Lysates (from left to right): Hela and A549 cell Amount of lysate: 40ug per lane Primary antibody: 1/500 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 minutes)

Testing Data (Gel: 8%SDS-PAGE Lysates (from left to right): Hela and A549 cell Amount of lysate: 40ug per lane Primary antibody: 1/500 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 minutes)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/40.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/40.)
Related Product Information for anti-NDUFA10 antibody
The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.
Product Categories/Family for anti-NDUFA10 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,563 Da
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
NCBI Official Symbol
NDUFA10
NCBI Official Synonym Symbols
CI-42k; CI-42KD
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
Protein Family
UniProt Gene Name
NDUFA10
UniProt Synonym Gene Names
CI-42kD
UniProt Entry Name
NDUAA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. [provided by RefSeq, Jul 2008]

Uniprot Description

NDUFA10: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA10 subunit family.

Protein type: EC 1.6.99.3; Oxidoreductase; Mitochondrial; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: mitochondrial matrix; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: nucleoside kinase activity; NADH dehydrogenase (ubiquinone) activity; ATP binding

Biological Process: response to drug; cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; nucleotide biosynthetic process

Disease: Leigh Syndrome

Research Articles on NDUFA10

Similar Products

Product Notes

The NDUFA10 ndufa10 (Catalog #AAA9409430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFA10 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFA10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:200-1:1000 Immunohistochemistry: 1:50-1:200. Researchers should empirically determine the suitability of the NDUFA10 ndufa10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFA10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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