Rabbit EIF4A1 Polyclonal Antibody | anti-EIF4A1 antibody

EIF4A1 Antibody

Cat. Num. AAA9408770

• Gene Names: EIF4A1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: EIF4A1; Polyclonal Antibody; EIF4A1 Antibody; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I; anti-EIF4A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total EIF4A1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 347

• Applicable Applications for anti-EIF4A1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human EIF4A1.
• Target Name: EIF4A1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using EIF4A1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung using EIF4A1 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat lung using EIF4A1 antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cell using EIF4A1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-EIF4A1 antibody

EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Product Categories/Family for anti-EIF4A1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 325197164
• NCBI GeneID: 1973
• NCBI Accession #: NP_001191439.1
• NCBI GenBank Nucleotide #: NM_001204510.1
• UniProt Accession #: P60842; P04765; Q5U018; Q61516; B2R6L8; D3DTP9; J3QLC4
• Molecular Weight: 39,548 Da
• NCBI Official Full Name: eukaryotic initiation factor 4A-I isoform 2
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4A1
• NCBI Official Symbol: EIF4A1
• NCBI Official Synonym Symbols: DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
• NCBI Protein Information: eukaryotic initiation factor 4A-I
• UniProt Protein Name: Eukaryotic initiation factor 4A-I
• Protein Family: Eukaryotic initiation factor
• UniProt Gene Name: EIF4A1
• UniProt Synonym Gene Names: DDX2A; EIF4A; eIF-4A-I; eIF4A-I
• UniProt Entry Name: IF4A1_HUMAN

Uniprot Description

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.