Western Blot (WB)
(Western blot analysis of NDUFA10 expression in Human fetal heart lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human NDUFA10 Polyclonal Antibody | anti-NDUFA10 antibody
NDUFA10 Antibody
Gene Names
NDUFA10; CI-42k; CI-42KD
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NDUFA10; Polyclonal Antibody; NDUFA10 Antibody; CI 42KD; CI-42k; Complex I-42kD; MGC5103; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 42kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10; mitochondrial; mitochondrial complex I 42kDa subunit; NADH ubiquinone oxidoreductase 42 kDa subunit mitochondrial precursor; NADH-ubiquinone oxidoreductase 42 kDa subunit; ndufa10l; anti-NDUFA10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
NDUFA10 antibody detects endogenous levels of total NDUFA10
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
355
Applicable Applications for anti-NDUFA10 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Mitochondrion Matrix.
Predicted Cross Reactivity
Pig
Similarity
Pig (83%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of NDUFA10 expression in Human fetal heart lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of NDUFA10 expression in Human fetal heart lysate, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9603749 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603749 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NDUFA10 antibody
Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Subunit Structure: Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction.
Post-translational Modifications: Phosphorylation at Ser-250 by PINK1 is required for the binding and/or reduction of the complex I substrate ubiquinone.
Similarity: Belongs to the complex I NDUFA10 subunit family.
Subunit Structure: Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction.
Post-translational Modifications: Phosphorylation at Ser-250 by PINK1 is required for the binding and/or reduction of the complex I substrate ubiquinone.
Similarity: Belongs to the complex I NDUFA10 subunit family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 41 kDa
Predicted: 41 kDa
Predicted: 41 kDa
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit A10
NCBI Official Symbol
NDUFA10
NCBI Official Synonym Symbols
CI-42k; CI-42KD
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
Protein Family
UniProt Gene Name
NDUFA10
UniProt Synonym Gene Names
CI-42kD
NCBI Description
The protein encoded by this gene is a component of 42 kDa complex I, the first enzyme complex in the electron transport chain of mitochondria. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. A mutation in this gene was found in an individual with Leigh syndrome. [provided by RefSeq, Apr 2016]
Uniprot Description
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Research Articles on NDUFA10
1. NDUFA10 was found to be differentially methylated and expressed in human squamous cell carcinomas.
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Product Notes
The NDUFA10 ndufa10 (Catalog #AAA9603749) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFA10 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFA10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NDUFA10 ndufa10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFA10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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