Rabbit anti-Human, Mouse NDUFA10 Polyclonal Antibody | anti-NDUFA10 antibody

NDUFA10, ID (NDUFA10, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, Complex I-42kD, NADH-ubiquinone oxidoreductase 42kD subunit) (FITC)

Cat. Num. AAA6324356

• Gene Names: NDUFA10; CI-42k; CI-42KD
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NDUFA10; Polyclonal Antibody; ID (NDUFA10; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10; mitochondrial; Complex I-42kD; NADH-ubiquinone oxidoreductase 42kD subunit) (FITC); NADH-ubiquinone oxidoreductase 42kD subunit; anti-NDUFA10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NDUFA10 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NDUFA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the Central region of human NDUFA10.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NDUFA10 Antibody (Center) western blot analysis in HepG2 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NDUFA10 antibody detected the NDUFA10 protein (arrow).)

Immunohistochemistry (IHC)

(NDUFA10 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFA10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NDUFA10 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NDUFA10 antibody

The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.

Product Categories/Family for anti-NDUFA10 antibody

Antibodies; Mitochondrial Proteins

References

Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009). Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008). Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008). Ma, J., et al. Atherosclerosis 191(1):63-72(2007). Harris, S.E., et al. BMC Genet. 8, 43 (2007). Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4758768
• NCBI GeneID: 4705
• NCBI Accession #: NP_004535
• NCBI GenBank Nucleotide #: NM_004544
• UniProt Accession #: O95299; Q8WXC9
• Molecular Weight: 48,563 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
• NCBI Official Symbol: NDUFA10
• NCBI Official Synonym Symbols: CI-42k; CI-42KD
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; NADH-ubiquinone oxidoreductase 42 kDa subunit; complex I 42kDa subunit
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFA10
• UniProt Synonym Gene Names: CI-42kD
• UniProt Entry Name: NDUAA_HUMAN