Rabbit anti-Human CACNB2 Polyclonal Antibody | anti-CACNB2 antibody

CACNB2, ID (CACNB2, CACNLB2, MYSB, Voltage-dependent L-type calcium channel subunit beta-2, Calcium channel voltage-dependent subunit beta 2, Lambert-Eaton myasthenic syndrome antigen B) (FITC)

Cat. Num. AAA6280356

• Gene Names: CACNB2; MYSB; CAVB2; CACNLB2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CACNB2; Polyclonal Antibody; ID (CACNB2; CACNLB2; MYSB; Voltage-dependent L-type calcium channel subunit beta-2; Calcium channel voltage-dependent subunit beta 2; Lambert-Eaton myasthenic syndrome antigen B) (FITC); Lambert-Eaton myasthenic syndrome antigen B; anti-CACNB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CACNB2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CACNB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the Central region of human CACNB2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CACNB2 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CACNB2 antibody detected the CACNB2 protein (arrow).)

Related Product Information for anti-CACNB2 antibody

This gene encodes a subunit of a voltage-dependent calcium channel protein which is a member of the voltage-gated calcium channel superfamily. The gene product was originally identified as an antigen target in Lambert-Eaton myasthenic syndrome which is an autoimmune disorder. Mutations in this gene are associated with Brugada symdrome. Alternatively spliced variants have been identified for this gene.

Product Categories/Family for anti-CACNB2 antibody

Antibodies; Ion Channel

References

Burashnikov, E., et al. Heart Rhythm (2010). In press: Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Takeuchi, F., et al. Circulation 121(21):2302-2309(2010). Hong, K.W., et al. J. Hum. Genet. 55(6):336-341(2010). Lee, M.T., et al. Mol. Psychiatry (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 45359834
• NCBI GeneID: 783
• NCBI Accession #: NP_963864.1; NP_963890.2
• NCBI GenBank Nucleotide #: NM_201570.2
• UniProt Accession #: Q08289; AAB27916
• Molecular Weight: 68,838 Da
• NCBI Official Full Name: voltage-dependent L-type calcium channel subunit beta-2 isoform 7
• NCBI Official Synonym Full Names: calcium channel, voltage-dependent, beta 2 subunit
• NCBI Official Symbol: CACNB2
• NCBI Official Synonym Symbols: MYSB; CAVB2; CACNLB2
• NCBI Protein Information: voltage-dependent L-type calcium channel subunit beta-2; CAB2; lambert-Eaton myasthenic syndrome antigen B; myasthenic (Lambert-Eaton) syndrome antigen B; calcium channel voltage-dependent subunit beta 2
• UniProt Protein Name: Voltage-dependent L-type calcium channel subunit beta-2
• Protein Family: Voltage-dependent L-type calcium channel
• UniProt Gene Name: CACNB2
• UniProt Synonym Gene Names: CACNLB2; MYSB; CAB2; MYSB
• UniProt Entry Name: CACB2_HUMAN

NCBI Description

This gene encodes a subunit of a voltage-dependent calcium channel protein that is a member of the voltage-gated calcium channel superfamily. The gene product was originally identified as an antigen target in Lambert-Eaton myasthenic syndrome, an autoimmune disorder. Mutations in this gene are associated with Brugada syndrome. Alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Feb 2013]

Uniprot Description

CACNB2: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. Defects in CACNB2 are the cause of Brugada syndrome type 4 (BRGDA4). A heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the calcium channel beta subunit family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, calcium

Chromosomal Location of Human Ortholog: 10p12

Cellular Component: integral to plasma membrane; voltage-gated calcium channel complex; sarcolemma

Molecular Function: voltage-gated calcium channel activity; protein binding; calcium channel activity; high voltage-gated calcium channel activity

Biological Process: synaptic transmission; axon guidance; visual perception; transport; positive regulation of calcium ion transport; neuromuscular junction development

Disease: Brugada Syndrome 4