Rabbit anti-Human, Mouse NDUFA10 Polyclonal Antibody | anti-NDUFA10 antibody

NDUFA10, ID (NDUFA10, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, Complex I-42kD, NADH-ubiquinone oxidoreductase 42kD subunit)

Cat. Num. AAA6008347

• Gene Names: NDUFA10; CI-42k; CI-42KD
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NDUFA10; Polyclonal Antibody; ID (NDUFA10; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10; mitochondrial; Complex I-42kD; NADH-ubiquinone oxidoreductase 42kD subunit); Anti -NDUFA10; anti-NDUFA10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NDUFA10 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: NDUFA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the Central region of human NDUFA10.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NDUFA10 Antibody (Center) western blot analysis in HepG2 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NDUFA10 antibody detected the NDUFA10 protein (arrow).)

Immunohistochemistry (IHC)

(NDUFA10 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFA10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NDUFA10 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NDUFA10 antibody

The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.

Product Categories/Family for anti-NDUFA10 antibody

Antibodies; Abs to Mitochondrial Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4758768
• NCBI GeneID: 4705
• NCBI Accession #: NP_004535.1
• NCBI GenBank Nucleotide #: NM_004544.3
• UniProt Accession #: O95299
• Molecular Weight: 40,751 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
• NCBI Official Symbol: NDUFA10
• NCBI Official Synonym Symbols: CI-42k; CI-42KD
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; complex I 42kDa subunit; NADH-ubiquinone oxidoreductase 42 kDa subunit
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFA10
• UniProt Synonym Gene Names: CI-42kD
• UniProt Entry Name: NDUAA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. [provided by RefSeq, Jul 2008]

Uniprot Description

NDUFA10: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA10 subunit family.

Protein type: Oxidoreductase; Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 1.6.99.3; EC 1.6.5.3

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: mitochondrial matrix; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: nucleoside kinase activity; NADH dehydrogenase (ubiquinone) activity; ATP binding

Biological Process: response to drug; cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; nucleotide biosynthetic process

Disease: Leigh Syndrome