Rabbit anti-Human, Mouse NDUFA1 Polyclonal Antibody | anti-NDUFA1 antibody

NDUFA1 Antibody (Center)

Cat. Num. AAA9215938

• Gene Names: NDUFA1; MWFE; ZNF183; CI-MWFE
• Reactivity: Human, Mouse
• Applications: Western Blot
• Synonyms: NDUFA1; Polyclonal Antibody; NDUFA1 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; Complex I-MWFE; CI-MWFE; NADH-ubiquinone oxidoreductase MWFE subunit; anti-NDUFA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This NDUFA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 26-59 amino acids from the Central region of human NDUFA1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 70

• Applicable Applications for anti-NDUFA1 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-NDUFA1 Antibody (Center) at 1:2000 dilutionLane 1: A-673 whole cell lysateLane 2: human skeletal muscle lysateLane 3: mouse kidney lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 8 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NDUFA1 antibody

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Product Categories/Family for anti-NDUFA1 antibody

Crown Antibodies; Cancer; Metabolism; Signal Transduction

References

Zhuchenko O.,et al.Genomics 37:281-288(1996). Frattini A.,et al.Gene 192:291-298(1997). Zhuchenko O.P.,et al.Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases. Murray J.,et al.J. Biol. Chem. 278:13619-13622(2003). Sjoeblom T.,et al.Science 314:268-274(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4758770
• NCBI GeneID: 4694
• NCBI Accession #: NP_004532.1
• NCBI GenBank Nucleotide #: NM_004541.3
• UniProt Accession #: O15239
• Molecular Weight: 8072
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase subunit A1
• NCBI Official Symbol: NDUFA1
• NCBI Official Synonym Symbols: MWFE; ZNF183; CI-MWFE
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
• UniProt Gene Name: NDUFA1
• UniProt Synonym Gene Names: CI-MWFE
• UniProt Entry Name: NDUA1_HUMAN

NCBI Description

The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008]

Uniprot Description

NDUFA1: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFA1 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I NDUFA1 subunit family.

Protein type: Membrane protein, integral; Mitochondrial; Oxidoreductase; EC 1.6.99.3; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial membrane; integral to membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone

Disease: Mitochondrial Complex I Deficiency