Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Testing Data (10ng of rhNM23-H1 (NME1), 10ng of rhNM23-H2 (NME2), and lysates from human A431, HepG2 and K562 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to a PVDF membrane and immunoblotted with 0.5ug/ml of MBS632615)

Mouse anti-Human NME1 Monoclonal Antibody | anti-NME1 antibody

NME1 (Nucleoside Diphosphate Kinase A, Granzyme A-activated DNase, GAAD, Metastasis Inhibition Factor nm23, NB, NBS, NDPKA, NDPK-A, NDP Kinase A, NM23, nm23-H1, NM23-H1, Tumor Metastatic Process-associated Protein)

Gene Names
NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Synonyms
NME1; Monoclonal Antibody; NME1 (Nucleoside Diphosphate Kinase A; Granzyme A-activated DNase; GAAD; Metastasis Inhibition Factor nm23; NB; NBS; NDPKA; NDPK-A; NDP Kinase A; NM23; nm23-H1; NM23-H1; Tumor Metastatic Process-associated Protein); Anti -NME1 (Nucleoside Diphosphate Kinase A; anti-NME1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10K87
Specificity
Recognizes human NME1 at ~20kD. Does not crossreact with recombinant human NME2.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form/Format
Supplied as a lyophilized powder from PBS, pH7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Concentration
~0.5mg/mL (after reconstitution) (varies by lot)
Applicable Applications for anti-NME1 antibody
Western Blot (WB)
Application Notes
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Immunogen
Full length recombiant protein corresponding to Met1-Glu 152 from human NME2, expressed in E.col
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(10ng of rhNM23-H1 (NME1), 10ng of rhNM23-H2 (NME2), and lysates from human A431, HepG2 and K562 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to a PVDF membrane and immunoblotted with 0.5ug/ml of MBS632615)

Testing Data (10ng of rhNM23-H1 (NME1), 10ng of rhNM23-H2 (NME2), and lysates from human A431, HepG2 and K562 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to a PVDF membrane and immunoblotted with 0.5ug/ml of MBS632615)
Related Product Information for anti-NME1 antibody
NME1 (NM23-H1) is a 19-20kD member of the NDK family of enzymes. NME1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NME2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows His and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/mL concentrations. Human NME1 is 152aa in length, contains one NDP kinase domain (aa5-134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NME1 shares 89% aa identity with human 17-18kD NME2, and 94% aa identity with mouse NME1. A second H1 isoform named M23-H1B with 25 additional aa at the N-terminus has also been described.
Product Categories/Family for anti-NME1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nucleoside diphosphate kinase A isoform a
NCBI Official Synonym Full Names
NME/NM23 nucleoside diphosphate kinase 1
NCBI Official Symbol
NME1
NCBI Official Synonym Symbols
NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
NCBI Protein Information
nucleoside diphosphate kinase A; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; non-metastatic cells 1, protein (NM23A) expressed in
UniProt Protein Name
Nucleoside diphosphate kinase A
UniProt Gene Name
NME1
UniProt Synonym Gene Names
NDPKA; NM23; NDK A; NDP kinase A; GAAD
UniProt Entry Name
NDKA_HUMAN

NCBI Description

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported.

Protein type: Nucleotide Metabolism - purine; EC 2.7.4.6; Nucleotide Metabolism - pyrimidine; Tumor suppressor; Kinase, nucleoside diphosphate; Kinase, other; Protein kinase, histidine; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: mitochondrion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; GTP binding; nucleoside diphosphate kinase activity; magnesium ion binding; deoxyribonuclease activity; ribosomal small subunit binding; ATP binding

Biological Process: GTP biosynthetic process; nervous system development; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UTP biosynthetic process; nucleoside diphosphate phosphorylation; endocytosis; DNA catabolic process; regulation of apoptosis; negative regulation of cell proliferation; CTP biosynthetic process; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; cell differentiation; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; positive regulation of DNA binding

Disease: Neuroblastoma, Susceptibility To

Research Articles on NME1

Similar Products

Product Notes

The NME1 nme1 (Catalog #AAA632615) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NME1 (Nucleoside Diphosphate Kinase A, Granzyme A-activated DNase, GAAD, Metastasis Inhibition Factor nm23, NB, NBS, NDPKA, NDPK-A, NDP Kinase A, NM23, nm23-H1, NM23-H1, Tumor Metastatic Process-associated Protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NME1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the NME1 nme1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NME1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.