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Western Blot (WB) (Gel: 12%SDS-PAGE, Lysate: 30 ug, Lane: Mouse brain tissue, Primary antibody: 861015(NDRG3 Antibody) at dilution 1/1050, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds)

Rabbit anti-Human, Mouse NDRG3 Polyclonal Antibody | anti-NDRG3 antibody

NDRG3 Rabbit pAb

Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
NDRG3; Polyclonal Antibody; NDRG3 Rabbit pAb; NDRG family member 3; anti-NDRG3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-NDRG3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:1000-1:5000
IHC: 1:100-1:300
Immunogen
Synthetic peptide of human NDRG3
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Cytoplasm
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 30 ug, Lane: Mouse brain tissue, Primary antibody: 861015(NDRG3 Antibody) at dilution 1/1050, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds)

Western Blot (WB) (Gel: 12%SDS-PAGE, Lysate: 30 ug, Lane: Mouse brain tissue, Primary antibody: 861015(NDRG3 Antibody) at dilution 1/1050, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 861015(NDRG3 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 861015(NDRG3 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 861015(NDRG3 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human brain tissue using 861015(NDRG3 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: ×200))
Product Categories/Family for anti-NDRG3 antibody

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
41kDa
UniProt Protein Name
Protein NDRG3
Protein Family
UniProt Gene Name
NDRG3
UniProt Entry Name
NDRG3_HUMAN

Uniprot Description

NDRG3: sequence is similar to alpha/beta hydrolases. During development, NDRG3 is expressed at high levels in the spinal cord and the thymic rudiment. NDRG3 is located throughout the mouse cerebrum and cerebellum. NDRG1 and NDRG3 belong to a subfamily, and NDRG2 and NDRG4 to another. In humans, NDRG3 is highly expressed in brain and testis. Unlike Ndr1, Ndr2 and Ndr3 appear not to be controlled by N-myc or c-myc. Three alternately spliced human isoforms have been reported.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 20q11.21-q11.23

Cellular Component: cytoplasm

Biological Process: spermatogenesis; negative regulation of cell growth; cell differentiation

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Product Notes

The NDRG3 ndrg3 (Catalog #AAA4758342) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDRG3 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NDRG3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:1000-1:5000 IHC: 1:100-1:300. Researchers should empirically determine the suitability of the NDRG3 ndrg3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDRG3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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