Western Blot (WB)
(Western blot analysis of lysates from human liver, mouse liver tissue lysate (from left to right), using NCOA2 Antibody (C-term). MBS9213938 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Rabbit anti-Human, mouse NCOA2 Polyclonal Antibody | anti-NCOA2 antibody
NCOA2 Antibody (C-term)
Western Blot (WB)
(Western blot analysis of lysates from human liver, mouse liver tissue lysate (from left to right), using NCOA2 Antibody (C-term). MBS9213938 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from human liver, mouse liver tissue lysate (from left to right), using NCOA2 Antibody (C-term). MBS9213938 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Carapeti M.,et al.Blood 91:3127-3133(1998).
Voegel J.J.,et al.EMBO J. 17:507-519(1998).
Fryer C.J.,et al.Nature 393:88-91(1998).
Carrero P.,et al.Mol. Cell. Biol. 20:402-415(2000).
NCBI and Uniprot Product Information
NCBI Description
The NCOA2 gene encodes nuclear receptor coactivator 2, which aids in the function of nuclear hormone receptors. Nuclear hormone receptors are conditional transcription factors that play important roles in various aspects of cell growth, development, and homeostasis by controlling expression of specific genes. Members of the nuclear hormone receptor superfamily, which includes the 5 steroid receptors and class II nuclear receptors (see below), are structurally characterized by 3 distinct domains: an N-terminal transcriptional activation domain, a central DNA-binding domain, and a C-terminal hormone-binding domain. Before the binding of hormone, steroid receptors, which are sometimes called class I of the nuclear hormone receptor family, remain inactive in a complex with heat-shock protein-90 (MIM 140571) and other stress family proteins. Binding of hormone induces critical conformational changes in steroid receptors that cause them to dissociate from the inhibitory complex, bind as homodimers to specific DNA enhancer elements associated with target genes, and modulate that gene's transcription. After binding to enhancer elements, transcription factors require transcriptional coactivator proteins to mediate their stimulation of transcription initiation (Hong et al., 1997 [PubMed 9111344]).[supplied by OMIM, Nov 2010]
Uniprot Description
SRC-2: a transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Present in a complex with CARM1 and p300. Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts with HIF1A, NCOA1, APEX and NR3C1. Interacts with CASP8AP2. Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the MYST3-NCOA2 oncogene, which consists of the N-terminus part of MYST3/MOZ and the C-terminus part of NCOA2/TIF2. MYST3-NCOA2 binds to CREBBP and disrupts its function in transcription activation. Phosphorylated and potentiated by the p38 MAPK signaling cascade in vitro and in vivo.
Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8q13.3
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: ligand-dependent nuclear receptor binding; protein dimerization activity; signal transducer activity; protein binding; histone acetyltransferase activity; ligand-dependent nuclear receptor transcription coactivator activity; transcription coactivator activity; chromatin binding; transcription factor binding; thyroid hormone receptor coactivator activity; nuclear hormone receptor binding
Biological Process: intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; histone acetylation; circadian regulation of gene expression; cellular lipid metabolic process; cellular response to hormone stimulus
Research Articles on NCOA2
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Product Notes
The NCOA2 ncoa2 (Catalog #AAA9213938) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCOA2 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NCOA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:500-1:1000. Researchers should empirically determine the suitability of the NCOA2 ncoa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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