Western Blot (WB)
(Western blot analysis of NCoA2 in lysates of HeLa , using NCoA2 Antibody.)
Rabbit NCoA2 Polyclonal Antibody | anti-NCoA2 antibody
NCoA2 Antibody
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
ELISA (Peptide): 1:20000-1:40000
Western Blot (WB)
(Western blot analysis of NCoA2 in lysates of HeLa , using NCoA2 Antibody.)
Western Blot (WB)
(Western blot analysis of NCoA2 in lysates of HeLa , using NCoA2 Antibody.)
Subunit Structure: Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with ESR1, HIF1A, NCOA1, APEX, NR3C1, NR3C2, CARM1, RARA, and RXRA. Present in a complex containing CARM1 and EP300/P300. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with PSMB9 and DDX5. Interacts (via LXXLL 1, 2 and 3 motifs) with RORA and RORC (via AF-2 motif). Interacts with RWDD3. Interacts with CLOCK and ARNTL/BMAL1 (By similarity). Interacts with NR4A3; potentiates the activity of the NR4A3 (By similarity). Interacts with NR1H3 (PubMed:19481530).
Similarity: Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant. The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently. Belongs to the SRC/p160 nuclear receptor coactivator family.
NCBI and Uniprot Product Information
Predicted: 160 kDa
NCBI Description
The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Uniprot Description
SRC-2: a transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Present in a complex with CARM1 and p300. Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts with HIF1A, NCOA1, APEX and NR3C1. Interacts with CASP8AP2. Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the MYST3-NCOA2 oncogene, which consists of the N-terminus part of MYST3/MOZ and the C-terminus part of NCOA2/TIF2. MYST3-NCOA2 binds to CREBBP and disrupts its function in transcription activation. Phosphorylated and potentiated by the p38 MAPK signaling cascade in vitro and in vivo.
Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8q13.3
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: ligand-dependent nuclear receptor binding; protein dimerization activity; signal transducer activity; protein binding; histone acetyltransferase activity; ligand-dependent nuclear receptor transcription coactivator activity; transcription coactivator activity; chromatin binding; transcription factor binding; thyroid hormone receptor coactivator activity; nuclear hormone receptor binding
Biological Process: intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; cellular lipid metabolic process; histone acetylation; negative regulation of transcription from RNA polymerase II promoter; cellular response to hormone stimulus
Research Articles on NCoA2
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Product Notes
The NCoA2 ncoa2 (Catalog #AAA9611182) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCoA2 Antibody reacts with Human Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's NCoA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the NCoA2 ncoa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCoA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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