Mouse anti-Mouse, Human NCK1 Monoclonal Antibody | anti-NCK1 antibody

Anti-NCK1 Purified

Cat. Num. AAA1800590

• Gene Names: NCK1; NCK; nck-1; NCKalpha
• Reactivity: Mouse, Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: NCK1; Monoclonal Antibody; Anti-NCK1 Purified; NCK adaptor protein 1; NCK-1; NCKalpha; anti-NCK1 antibody

• Host: Mouse
• Reactivity: Mouse, Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: EM-06
• Specificity: The mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 377

• Applicable Applications for anti-NCK1 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen: Purified recombinant second SH3 domain of human Nck1 fused to GST
• Preparation and Storage: Store at 2-8 degree C.

Western Blot (WB)

(Western blotting analysis of NCK1 in whole cell lysate of mouse lymph node lymphocytes using the antibody EM-06.)

Related Product Information for anti-NCK1 antibody

NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.

NCBI and Uniprot Product Information

• NCBI GI #: 127962
• NCBI GeneID: 4690
• UniProt Accession #: P16333
• NCBI Official Full Name: Cytoplasmic protein NCK1
• NCBI Official Synonym Full Names: NCK adaptor protein 1
• NCBI Official Symbol: NCK1
• NCBI Official Synonym Symbols: NCK; nck-1; NCKalpha
• NCBI Protein Information: cytoplasmic protein NCK1
• UniProt Protein Name: Cytoplasmic protein NCK1
• Protein Family: Cytoplasmic protein
• UniProt Gene Name: NCK1
• UniProt Synonym Gene Names: NCK; Nck-1
• UniProt Entry Name: NCK1_HUMAN

NCBI Description

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]

Uniprot Description

NCK1: adapter protein containing Src homology 2 and 3 (SH2 and SH3) domains. Transduces signals from tyrosine-phosphorylated growth factor receptors to their cellular substrates.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: endoplasmic reticulum; cytoplasm; plasma membrane; ribosome; protein phosphatase type 1 complex; intercellular junction; vesicle membrane; cytosol; nucleus

Molecular Function: protein binding, bridging; protein domain specific binding; protein binding; receptor signaling complex scaffold activity; protein kinase inhibitor activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity; receptor tyrosine kinase binding; receptor binding

Biological Process: lamellipodium biogenesis; axon guidance; positive regulation of translation in response to stress; T cell activation; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; response to other organism; T cell receptor signaling pathway; substrate-bound cell migration, cell extension; positive regulation of actin filament polymerization; negative regulation of protein kinase activity; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; vascular endothelial growth factor receptor signaling pathway