Rabbit anti-Human NCDN Polyclonal Antibody | anti-NCDN antibody

NCDN, NT (NCDN, KIAA0607, Neurochondrin) (Azide free) (HRP)

Cat. Num. AAA6324104

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NCDN; Polyclonal Antibody; NT (NCDN; KIAA0607; Neurochondrin) (Azide free) (HRP); Neurochondrin; anti-NCDN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NCDN antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NCDN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-144 amino acids from the N-terminal region of human NCDN.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NCDN Antibody (N-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the NCDN antibody detected the NCDN protein (arrow).)

Related Product Information for anti-NCDN antibody

This gene encodes a leucine-rich cytoplasmic protein, which is highly similar to a mouse protein that negatively regulates Ca/calmodulin-dependent protein kinase II phosphorylation and may be essential for spatial learning processes. Several alternatively spliced transcript variants of this gene have been described.

Product Categories/Family for anti-NCDN antibody

Antibodies; KIAA; orf

References

Dateki, M., et al. J. Biol. Chem. 280(21):20503-20508(2005). Mochizuki, R., et al. Biochem. Biophys. Res. Commun. 310(4):1219-1226(2003). Tchernev, V.T., et al. Mol. Med. 8(1):56-64(2002). Ohoka, Y., et al. Biochem. Biophys. Res. Commun. 280(1):237-243(2001). Mochizuki, R., et al. Biochim. Biophys. Acta 1490(3):367-368(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 62526031
• NCBI GeneID: 23154
• NCBI Accession #: NP_001014841.1; NP_055099.1
• NCBI GenBank Nucleotide #: NM_001014841.1
• UniProt Accession #: Q9UBB6; Q9UBY2; Q9Y4A6; Q9Y4D9; D3DPR9
• Molecular Weight: 78,864 Da
• NCBI Official Full Name: neurochondrin isoform 2
• NCBI Official Synonym Full Names: neurochondrin
• NCBI Official Symbol: NCDN
• NCBI Protein Information: neurochondrin
• UniProt Protein Name: Neurochondrin
• Protein Family: Neurochondrin
• UniProt Gene Name: NCDN
• UniProt Synonym Gene Names: KIAA0607

Uniprot Description

Probably involved in signal transduction, in the nervous system, via increasing cell surface localization of GRM5 and positively regulating its signaling (). Required for the spatial learning process. Acts as a negative regulator of Ca2+-calmodulin-dependent protein kinase 2 (CaMK2) phosphorylation. May play a role in modulating melanin-concentrating hormone-mediated functions via its interaction with MCHR1 that interferes with G protein-coupled signal transduction. May be involved in bone metabolism. May also be involved in neurite outgrowth.