Immunofluorescence (IF)
((0.5ug/ml) 24h-staining of PFA-perfused cryosection of Mouse Olfactory bub. Antigen retrieval with methanol (-20C, 10min) followed by 1% SDS(10min), IF-staning. Data obtained from anonymous customer.)
Goat NCAM2/OCAM Polyclonal Antibody | anti-NCAM2 antibody
Goat anti-NCAM2/OCAM Antibody
Gene Names
NCAM2; NCAM21
Reactivity
Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
NCAM2/OCAM; Polyclonal Antibody; Goat anti-NCAM2/OCAM Antibody; NCAM2; neural cell adhesion molecule 2; HGNC: 7657; MGC51008; NCAM21; OCAM; NCAM2 antibody; neural cell adhesion molecule 2 antibody; HGNC: 7657 antibody; MGC51008 antibody; NCAM21 antibody; OCAM antibody; NCAM2 / OCAM; anti-NCAM2 antibody
Host
Goat
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence
KVSNDIIQSKEDDSK
Sequence Length
837
Applicable Applications for anti-NCAM2 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Preliminary experiments gave an approx 150 and 28kDa bands in Human and Mouse Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93. 0kDa according to NP_004531.2. Both observed bands were successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry: Frozen section of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles (right side middle, figure 1) and moderate peripheral staining outside the glomeruli (top, figure 1). NCAM2 (red) is stained complementary to VGLUT2 (blue) and NQO1 (green) in figure 2. Recommended concentration:0.5 ug/mL. Paraffin embedded Human Brain (Cortex). Recommended concentration is: 3.75ug/ml.
Western Blot: Preliminary experiments gave an approx 150 and 28kDa bands in Human and Mouse Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93. 0kDa according to NP_004531.2. Both observed bands were successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry: Frozen section of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles (right side middle, figure 1) and moderate peripheral staining outside the glomeruli (top, figure 1). NCAM2 (red) is stained complementary to VGLUT2 (blue) and NQO1 (green) in figure 2. Recommended concentration:0.5 ug/mL. Paraffin embedded Human Brain (Cortex). Recommended concentration is: 3.75ug/ml.
Immunogen
Peptide with sequence C-KVSNDIIQSKEDDSK, from the C Terminus of the protein sequence according to NP_004531.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence (IF)
((0.5ug/ml) 24h-staining of PFA-perfused cryosection of Mouse Olfactory bub. Antigen retrieval with methanol (-20C, 10min) followed by 1% SDS(10min), IF-staning. Data obtained from anonymous customer.)
Immunofluorescence (IF)
((0.5ug/ml) 24h-staining of PFA-perfused cryosection of Mouse Olfactory bub. Antigen retrieval with methanol (-20C, 10min) followed by 1% SDS(10min), IF-staning. Data obtained from anonymous customer.)
Immunofluorescence (IF)
((0.5ug/ml) 48h-staining of PFA-perfused cryosection of Mouse Olfactory bulb. Antigen retrieval with citrate buffer pH 6 at 95C for 10min, IF-staning for NCAM2 (red), VGLUT2 (blue) and NQO1 (green), data obtained from anonymus customer.)
Immunofluorescence (IF)
((0.5ug/ml) 48h-staining of PFA-perfused cryosection of Mouse Olfactory bulb. Antigen retrieval with citrate buffer pH 6 at 95C for 10min, IF-staning for NCAM2 (red), VGLUT2 (blue) and NQO1 (green), data obtained from anonymus customer.)
Testing Data(TD)
((3.75 ug/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH, AP-staining.)
Testing Data(TD)
((3.75 ug/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
neural cell adhesion molecule 2
NCBI Official Synonym Full Names
neural cell adhesion molecule 2
NCBI Official Symbol
NCAM2
NCBI Official Synonym Symbols
NCAM21
NCBI Protein Information
neural cell adhesion molecule 2
UniProt Protein Name
Neural cell adhesion molecule 2
Protein Family
UniProt Gene Name
NCAM2
UniProt Synonym Gene Names
NCAM21; N-CAM-2; NCAM-2
UniProt Entry Name
NCAM2_HUMAN
NCBI Description
The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein and may function in selective fasciculation and zone-to-zone projection of the primary olfactory axons. [provided by RefSeq, Jul 2008]
Uniprot Description
NCAM2: May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 21q21.1
Cellular Component: axon; integral to membrane; plasma membrane
Biological Process: neuron adhesion
Research Articles on NCAM2
Similar Products
Product Notes
The NCAM2 ncam2 (Catalog #AAA421506) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NCAM2/OCAM Antibody reacts with Tested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's NCAM2/OCAM can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Preliminary experiments gave an approx 150 and 28kDa bands in Human and Mouse Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93. 0kDa according to NP_004531.2. Both observed bands were successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: Frozen section of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles (right side middle, figure 1) and moderate peripheral staining outside the glomeruli (top, figure 1). NCAM2 (red) is stained complementary to VGLUT2 (blue) and NQO1 (green) in figure 2. Recommended concentration:0.5 ug/mL. Paraffin embedded Human Brain (Cortex). Recommended concentration is: 3.75ug/ml. . Researchers should empirically determine the suitability of the NCAM2 ncam2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KVSNDIIQSK EDDSK. It is sometimes possible for the material contained within the vial of "NCAM2/OCAM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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