Western Blot (WB)
(Western blot analysis of NCALD expression in HeLa (A), NS-1 (B), H9C2 (C) whole cell lysates.)
Rabbit NCALD Polyclonal Antibody | anti-NCALD antibody
Anti-NCALD Antibody
Western Blot (WB)
(Western blot analysis of NCALD expression in HeLa (A), NS-1 (B), H9C2 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of NCALD expression in HeLa (A), NS-1 (B), H9C2 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NCALD staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NCALD staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
NCALD: May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions. Belongs to the recoverin family.
Chromosomal Location of Human Ortholog: 8q22.2
Cellular Component: clathrin coat of trans-Golgi network vesicle; intracellular; cytosol
Molecular Function: tubulin binding; clathrin binding; protein binding; calcium ion binding; actin binding; alpha-tubulin binding
Biological Process: vesicle-mediated transport; synaptic transmission; regulation of systemic arterial blood pressure; calcium-mediated signaling
Research Articles on NCALD
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Product Notes
The NCALD ncald (Catalog #AAA827514) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NCALD Antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's NCALD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the NCALD ncald for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCALD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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