Flow Cytometry (FC/FACS)
(Flow cytometric analysis of PFA-fixed HeLa cells. CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Mouse CREB5/CRE-BPa Monoclonal Antibody | anti-CREB5 antibody
CREB5/CRE-BPa Mouse Monoclonal Antibody [Clone PCRP-CREB5-1G8]
Gene Names
CREB5; CRE-BPA
Reactivity
HumanPredicted to work with Mouse and Rat.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Synonyms
CREB5/CRE-BPa; Monoclonal Antibody; CREB5/CRE-BPa Mouse Monoclonal Antibody [Clone PCRP-CREB5-1G8]; cAMP response element-binding protein CREBPa (CRE-BPa); cAMP responsive element binding protein 5 isoform alpha; CRE BPA; CREBPA; cyclic AMP responsive element binding protein 5; anti-CREB5 antibody
Host
Mouse
Reactivity
Human
Predicted to work with Mouse and Rat.
Predicted to work with Mouse and Rat.
Clonality
Monoclonal
Isotype
Mouse/IgG2b, kappa
Clone Number
PCRP-CREB5-1G8
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-CREB5 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
WB: 1-2ug/ml
IF: 1-2ug/ml
WB: 1-2ug/ml
Immunogen
Recombinant full-length human CREB5 protein
Cellular Localization
Nucleus
Positive Control
HeLa, U87 or K562 cells.
Hu-chromosome Location
7p15.1
Unigene
437075
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of PFA-fixed HeLa cells. CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of PFA-fixed HeLa cells. CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Related Product Information for anti-CREB5 antibody
The ATF/CREB family consists of a series of transcription factors that function by binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB-3 and Luman), CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. These transcription factors share terminal leucine zipper dimerization and basic DNA binding domains and are highly variable in their N-termini. Although each of the ATF/ CREB proteins bind CREs in their homodimeric form, they can also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. Protein kinase A- mediated CREB phosphorylation induces association with a nuclear protein designated CBP (CREB-binding protein), which may represent a CREB coactivator. CRE-BPa is a nuclear protein that binds DNA as a homodimer but can also form a heterodimer with ATF-2 or Jun.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
508
NCBI Official Full Name
Cyclic AMP-responsive element-binding protein 5
NCBI Official Synonym Full Names
cAMP responsive element binding protein 5
NCBI Official Symbol
CREB5
NCBI Official Synonym Symbols
CRE-BPA
NCBI Protein Information
cyclic AMP-responsive element-binding protein 5; CREB-5; H_GS165L15.1; cAMP-responsive element-binding protein 5; cAMP response element binding protein CRE-Bpa; cAMP response element-binding protein CRE-BPa; cAMP responsive element binding protein 5 isofo
UniProt Protein Name
Cyclic AMP-responsive element-binding protein 5
Protein Family
UniProt Gene Name
CREB5
UniProt Synonym Gene Names
CREBPA; CREB-5; cAMP-responsive element-binding protein 5
UniProt Entry Name
CREB5_HUMAN
NCBI Description
The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
CREB5: Binds to the cAMP response element and activates transcription. Belongs to the bZIP family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 7p15.1
Cellular Component: nucleus
Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; transcription factor activity
Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
Research Articles on CREB5
Similar Products
Product Notes
The CREB5 creb5 (Catalog #AAA4382404) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CREB5/CRE-BPa Mouse Monoclonal Antibody [Clone PCRP-CREB5-1G8] reacts with Human Predicted to work with Mouse and Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's CREB5/CRE-BPa can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC/FACS: 1-2ug/million cells IF: 1-2ug/ml WB: 1-2ug/ml. Researchers should empirically determine the suitability of the CREB5 creb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CREB5/CRE-BPa, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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