Western Blot (WB)
(NAT14 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).)
Rabbit anti-Human, Mouse NAT14 Polyclonal Antibody | anti-NAT14 antibody
NAT14, NT (NAT14, KLP1, N-acetyltransferase 14, K562 cell-derived leucine-zipper-like protein 1) (Azide free) (HRP)
Gene Names
NAT14; KLP1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NAT14; Polyclonal Antibody; NT (NAT14; KLP1; N-acetyltransferase 14; K562 cell-derived leucine-zipper-like protein 1) (Azide free) (HRP); K562 cell-derived leucine-zipper-like protein 1; anti-NAT14 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-NAT14 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
NAT14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from the N-terminal region of human NAT14.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(NAT14 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).)
Western Blot (WB)
(NAT14 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).)
Immunohistochemistry (IHC)
(NAT14 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NAT14 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(NAT14 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NAT14 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-NAT14 antibody
References
Wiame, E., et al. Biochem. J. 425(1):127-136(2010). Lamesch, P., et al. Genomics 89(3):307-315(2007). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Takahashi, S., et al. Biochim. Biophys. Acta 1522(3):207-211(2001). Takahashi, S., et al. Biochem. Biophys. Res. Commun. 273(2):596-602(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,650 Da
NCBI Official Full Name
N-acetyltransferase 14
NCBI Official Synonym Full Names
N-acetyltransferase 14 (GCN5-related, putative)
NCBI Official Symbol
NAT14
NCBI Official Synonym Symbols
KLP1
NCBI Protein Information
N-acetyltransferase 14; K562 cell-derived leucine-zipper-like protein 1; K562 cells-derived leucine zipper-like protein 1; K562 cells-derived leucine-zipper-like protein 1
UniProt Protein Name
N-acetyltransferase 14
Protein Family
UniProt Gene Name
NAT14
UniProt Synonym Gene Names
KLP1
UniProt Entry Name
NAT14_HUMAN
Uniprot Description
NAT14: Probable acetyltransferase that binds the 5'-GGACTACAG- 3' sequence of coproporphyrinogen oxidase promoter. Able to activate transcription of a reporter construct in vitro. Belongs to the camello family.
Protein type: Membrane protein, integral; Acetyltransferase; EC 2.3.1.-
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: integral to membrane; nucleus
Molecular Function: N-acetyltransferase activity; DNA binding
Biological Process: positive regulation of transcription, DNA-dependent; transcription initiation
Research Articles on NAT14
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Product Notes
The NAT14 nat14 (Catalog #AAA6323917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NAT14, NT (NAT14, KLP1, N-acetyltransferase 14, K562 cell-derived leucine-zipper-like protein 1) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NAT14 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NAT14 nat14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAT14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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