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Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
anti-Human EPI64/TBC1D10A Antibody | anti-TBC1D10A antibody
Goat anti-EPI64/TBC1D10A Antibody
Gene Names
TBC1D10A; EPI64; TBC1D10; dJ130H16.1; dJ130H16.2
Reactivity
Human
Applications
ELISA, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
EPI64/TBC1D10A; Goat anti-EPI64/TBC1D10A Antibody; EPI64; EPI 64; dJ130H16.. TBC1D10; TBC1 domain family member 10A; Rab27A-GAP-alpha; EBP50-PDZ interactor of 64 kD; ; anti-TBC1D10A antibody
Reactivity
Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
508
Applicable Applications for anti-TBC1D10A antibody
Peptide ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:2000.
IF: Strong expression of the protein seen in the cytoplasm/ plasma membrane and nucleus of MCF7 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
IF: Strong expression of the protein seen in the cytoplasm/ plasma membrane and nucleus of MCF7 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
Immunogen Sequence
Peptide with sequence AKSNGENGPRAPA-C, from the N Terminus of the protein sequence according to NP_114143; NP_001191169.1.
Peptide Sequence + C
AKSNGENGPRAPA-C
Epitope
N Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Immunohistochemistry (IHC)
((4.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.)
Immunohistochemistry (IHC)
((4.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.)
Immunohistochemistry (IHC)
((4.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.This data is from a previous batch, not on sale.)
Immunohistochemistry (IHC)
((4.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.This data is from a previous batch, not on sale.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,935 Da
NCBI Official Full Name
TBC1 domain family member 10A isoform 2
NCBI Official Synonym Full Names
TBC1 domain family member 10A
NCBI Official Symbol
TBC1D10A
NCBI Official Synonym Symbols
EPI64; TBC1D10; dJ130H16.1; dJ130H16.2
NCBI Protein Information
TBC1 domain family member 10A
UniProt Protein Name
TBC1 domain family member 10A
Protein Family
UniProt Gene Name
TBC1D10A
UniProt Synonym Gene Names
EPI64; TBC1D10; EPI64 protein
Uniprot Description
Acts as GTPase-activating protein for RAB27A, but not for RAB2A, RAB3A, nor RAB4A.
Research Articles on TBC1D10A
Similar Products
Product Notes
The TBC1D10A tbc1d10a (Catalog #AAA423698) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EPI64/TBC1D10A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EPI64/TBC1D10A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Pep-ELISA: antibody detection limit dilution 1:2000. IF: Strong expression of the protein seen in the cytoplasm/ plasma membrane and nucleus of MCF7 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the TBC1D10A tbc1d10a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPI64/TBC1D10A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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