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Western Blot (WB) (Western blot analysis of extracts of HepG2 cells, using NAPRT1 antibody.)

Rabbit NAPRT1 Polyclonal Antibody | anti-NAPRT1 antibody

NAPRT1 Antibody

Gene Names
NAPRT; NAPRT1; PP3856
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NAPRT1; Polyclonal Antibody; NAPRT1 Antibody; FHA HIT interacting protein; FHA-HIT-interacting protein; FHIP; NAPRTase; Nicotinate phosphoribosyltransferase; Nicotinate phosphoribosyltransferase domain containing 1; Nicotinate phosphoribosyltransferase domain-containing protein 1; Nicotinic acid phosphoribosyltransferase; PNCB_HUMAN; PP3856; anti-NAPRT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NAPRT1 antibody detects endogenous levels of NAPRT1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
525
Applicable Applications for anti-NAPRT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human NAPRT1.
Subcellular Location
Cytoplasm > Cytosol.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (92%), Bovine (100%), Horse (92%), Sheep (100%), Dog (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of HepG2 cells, using NAPRT1 antibody.)

Western Blot (WB) (Western blot analysis of extracts of HepG2 cells, using NAPRT1 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from various samples, using NAPRT1 antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Hepg2; )

Western Blot (WB) (Western blot analysis of extracts from various samples, using NAPRT1 antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Hepg2; )

Immunohistochemistry (IHC)

(MBS9603443 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9603443 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NAPRT1 antibody
Function: Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells.
Similarity: Belongs to the NAPRTase family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 51 kDa; 56 kDa
Predicted: 58 kDa
NCBI Official Full Name
nicotinate phosphoribosyltransferase isoform 2
NCBI Official Synonym Full Names
nicotinate phosphoribosyltransferase
NCBI Official Symbol
NAPRT
NCBI Official Synonym Symbols
NAPRT1; PP3856
NCBI Protein Information
nicotinate phosphoribosyltransferase
UniProt Protein Name
Nicotinate phosphoribosyltransferase
UniProt Gene Name
NAPRT
UniProt Synonym Gene Names
FHIP; NAPRT1; NAPRTase

NCBI Description

Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM, Mar 2008]

Uniprot Description

Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed:17604275, PubMed:21742010, PubMed:26042198). Helps prevent cellular oxidative stress via its role in NAD biosynthesis (PubMed:17604275).

Research Articles on NAPRT1

Similar Products

Product Notes

The NAPRT1 naprt (Catalog #AAA9603443) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NAPRT1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NAPRT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NAPRT1 naprt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAPRT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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