Western Blot (WB)
(Western blot analysis of extracts of mouse thymus tissue lysate, using NAP1L1 antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit NAP1L1 Polyclonal Antibody | anti-NAP1L1 antibody
NAP1L1 Antibody
Gene Names
NAP1L1; NRP; NAP1; NAP1L
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NAP1L1; Polyclonal Antibody; NAP1L1 Antibody; FLJ16112; hNRP; HSP22 like protein interacting protein; MGC23410; MGC8688; NAP 1; NAP 1 related protein; NAP 1L; NAP-1-related protein; NAP1; NAP1 L1; NAP1 related protein; NAP1L; Nap1l1; NAP1L1 protein; NP1L1_HUMAN; NRP; Nucleosome assembly protein 1 like 1; Nucleosome assembly protein 1-like 1; Nucleosome assembly protein I-related protein; anti-NAP1L1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NAP1L1 antibody detects endogenous levels of total NAP1L1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
383
Applicable Applications for anti-NAP1L1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human NAP1L1
Subcellular Location
Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity
Ubiquitously expressed.
Predicted Cross Reactivity
Bovine, Horse, Rabbit, Dog, Chicken
Similarity
Bovine (100%), Horse (92%), Rabbit (100%), Dog (92%), Chicken (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of mouse thymus tissue lysate, using NAP1L1 antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts of mouse thymus tissue lysate, using NAP1L1 antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9607729 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9607729 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NAP1L1 antibody
Description: This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing of this gene results in several transcript variants; however, not all have been fully described.
Function: May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
Post-translational Modifications: Monoglycylated on glutamate residues. Cannot be polyglycylated due to the absence of functional TTLL10 in human (By similarity). Polyglutamylated by TTLL4 on glutamate residues, resulting in polyglutamate chains on the gamma-carboxyl group. Both polyglutamylation and monoglycylation modifications can coexist on the same protein on adjacent residues, and lowering polyglycylation levels increases polyglutamylation, and reciprocally.
Similarity: The acidic domains are probably involved in the interaction with histones. Belongs to the nucleosome assembly protein (NAP) family.
Function: May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
Post-translational Modifications: Monoglycylated on glutamate residues. Cannot be polyglycylated due to the absence of functional TTLL10 in human (By similarity). Polyglutamylated by TTLL4 on glutamate residues, resulting in polyglutamate chains on the gamma-carboxyl group. Both polyglutamylation and monoglycylation modifications can coexist on the same protein on adjacent residues, and lowering polyglycylation levels increases polyglutamylation, and reciprocally.
Similarity: The acidic domains are probably involved in the interaction with histones. Belongs to the nucleosome assembly protein (NAP) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 45 kDa
Predicted: 46 kDa
Predicted: 46 kDa
NCBI Official Full Name
nucleosome assembly protein 1-like 1 isoform 2
NCBI Official Synonym Full Names
nucleosome assembly protein 1 like 1
NCBI Official Symbol
NAP1L1
NCBI Official Synonym Symbols
NRP; NAP1; NAP1L
NCBI Protein Information
nucleosome assembly protein 1-like 1
UniProt Protein Name
Nucleosome assembly protein 1-like 1
Protein Family
UniProt Gene Name
NAP1L1
UniProt Synonym Gene Names
NRP; hNRP
NCBI Description
This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms; however, not all have been fully described. [provided by RefSeq, Apr 2015]
Uniprot Description
Plays a key role in the regulation of embryonic neurogenesis (). Promotes the proliferation of neural progenitors and inhibits neuronal differentiation during cortical development (). Regulates neurogenesis via the modulation of RASSF10; regulates RASSF10 expression by promoting SETD1A-mediated H3K4 methylation at the RASSF10 promoter ().
Research Articles on NAP1L1
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Product Notes
The NAP1L1 nap1l1 (Catalog #AAA9607729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NAP1L1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NAP1L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NAP1L1 nap1l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAP1L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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