Rabbit anti-Human NANOS2 Polyclonal Antibody | anti-NANOS2 antibody

NANOS2, ID (NANOS2, NOS2, Nanos homolog 2) (AP)

Cat. Num. AAA6323737

• Gene Names: NANOS2; NOS2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NANOS2; Polyclonal Antibody; ID (NANOS2; NOS2; Nanos homolog 2) (AP); Nanos homolog 2; anti-NANOS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NANOS2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NANOS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the Central region of human NANOS2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NANOS2 (arrow) using rabbit polyclonal NANOS2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NANOS2 gene.)

Related Product Information for anti-NANOS2 antibody

NANOS2 is required to support proliferation and self-renewal of proximal germ cells in males only. Probably regulates translation of specific mRNAs by associating with the 3'-UTR of mRNA targets. Essential for spermatogonia formation (By similarity).

Product Categories/Family for anti-NANOS2 antibody

Antibodies; Spermatogenesis; Testis Proteins

References

Kusz, K.M., et al. Mol. Hum. Reprod. 15(3):165-171(2009). Chang, H.R., et al. Arch. Dermatol. Res. 295(7):293-296(2003). Tsuda, M., et al. Science 301(5637):1239-1241(2003). Jaruzelska, J., et al. Dev. Genes Evol. 213(3):120-126(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 71143121
• NCBI GeneID: 339345
• NCBI Accession #: NP_001025032.1
• NCBI GenBank Nucleotide #: NM_001029861.2
• UniProt Accession #: P60321; Q17R30; Q4G0P8
• Molecular Weight: 15,132 Da
• NCBI Official Full Name: nanos homolog 2
• NCBI Official Synonym Full Names: nanos homolog 2 (Drosophila)
• NCBI Official Symbol: NANOS2
• NCBI Official Synonym Symbols: NOS2
• NCBI Protein Information: nanos homolog 2; NOS-2
• UniProt Protein Name: Nanos homolog 2
• Protein Family: Nanos
• UniProt Gene Name: NANOS2
• UniProt Synonym Gene Names: NOS2; NOS-2
• UniProt Entry Name: NANO2_HUMAN

Uniprot Description

NANOS2: Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state. Belongs to the nanos family.

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: mRNA binding; protein binding; zinc ion binding

Biological Process: multicellular organismal development; negative regulation of translation; negative regulation of meiosis; mRNA catabolic process; spermatogenesis; germ-line stem cell maintenance