Western Blot (WB)
(NOLC1 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NOLC1 antibody detected the NOLC1 protein (arrow).)
Rabbit anti-Human NOLC1 Polyclonal Antibody | anti-NOLC1 antibody
NOLC1, NT (NOLC1, KIAA0035, NS5ATP13, Nucleolar and coiled-body phosphoprotein 1, 140kD nucleolar phosphoprotein, Hepatitis C virus NS5A-transactivated protein 13, Nucleolar 130kD protein, Nucleolar phosphoprotein p130) (FITC)
Gene Names
NOLC1; P130; NOPP130; NOPP140; NS5ATP13
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NOLC1; Polyclonal Antibody; NT (NOLC1; KIAA0035; NS5ATP13; Nucleolar and coiled-body phosphoprotein 1; 140kD nucleolar phosphoprotein; Hepatitis C virus NS5A-transactivated protein 13; Nucleolar 130kD protein; Nucleolar phosphoprotein p130) (FITC); Nucleolar phosphoprotein p130; anti-NOLC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-NOLC1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
NOLC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-191 amino acids from the N-terminal region of human NOLC1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(NOLC1 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NOLC1 antibody detected the NOLC1 protein (arrow).)
Western Blot (WB)
(NOLC1 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NOLC1 antibody detected the NOLC1 protein (arrow).)
Related Product Information for anti-NOLC1 antibody
Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.
Product Categories/Family for anti-NOLC1 antibody
References
Thiry, M., et al. Histochem. Cell Biol. 132(2):129-140(2009). Hwang, Y.C., et al. Am. J. Pathol. 175(1):342-354(2009). Renvoise, B., et al. Hum. Mol. Genet. 18(7):1181-1189(2009). Lee, W.K., et al. Biochem. Biophys. Res. Commun. 376(2):439-444(2008). Tsai, Y.T., et al. J. Biomed. Sci. 15(4):471-486(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,603 Da
NCBI Official Full Name
nucleolar and coiled-body phosphoprotein 1
NCBI Official Synonym Full Names
nucleolar and coiled-body phosphoprotein 1
NCBI Official Symbol
NOLC1
NCBI Official Synonym Symbols
P130; NOPP130; NOPP140; NS5ATP13
NCBI Protein Information
nucleolar and coiled-body phosphoprotein 1; nucleolar protein p130; nucleolar 130 kDa protein; nucleolar phosphoprotein p130; 140 kDa nucleolar phosphoprotein; HCV NS5A-transactivated protein 13; HCV NS5A trans-regulated protein 13; nucleolar and coiled-b
UniProt Protein Name
Nucleolar and coiled-body phosphoprotein 1
Protein Family
UniProt Gene Name
NOLC1
UniProt Synonym Gene Names
KIAA0035; NS5ATP13; Nopp140
UniProt Entry Name
NOLC1_HUMAN
Uniprot Description
NOLC1: Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Nucleolus; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10q24.32
Cellular Component: nucleoplasm; Cajal body; cytoplasm; nucleolus
Molecular Function: protein binding; GTP binding; ATP binding
Biological Process: mitosis; cell cycle; rRNA processing; nucleolus organization and biogenesis
Research Articles on NOLC1
Similar Products
Product Notes
The NOLC1 nolc1 (Catalog #AAA6325975) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOLC1, NT (NOLC1, KIAA0035, NS5ATP13, Nucleolar and coiled-body phosphoprotein 1, 140kD nucleolar phosphoprotein, Hepatitis C virus NS5A-transactivated protein 13, Nucleolar 130kD protein, Nucleolar phosphoprotein p130) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOLC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NOLC1 nolc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOLC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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