Typical Testing Data/Standard Curve (for reference only)
Rat PHB (Prohibitin) CLIA Kit | PHB clia kit
Rat PHB (Prohibitin) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PHB. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PHB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PHB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PHB. You can calculate the concentration of PHB in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer.
Protein type: Transcription, coactivator/corepressor; Mitochondrial; Chaperone; Apoptosis; Motility/polarity/chemotaxis; Oncoprotein; Nuclear receptor co-regulator; Cell cycle regulation
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: nucleoplasm; cell surface; mitochondrion; membrane; integral to plasma membrane; mitochondrial inner membrane; early endosome; cytoplasm; plasma membrane; nucleus; myelin sheath
Molecular Function: protein C-terminus binding; protein binding; enzyme binding; proteinase activated receptor binding; histone deacetylase binding; complement component C3a binding; complement component C3b binding
Biological Process: mitochondrion organization and biogenesis; progesterone receptor signaling pathway; protein stabilization; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; signal transduction; regulation of apoptosis; osteoblast differentiation; negative regulation of cell proliferation; positive regulation of complement activation; regulation of transcription, DNA-dependent; positive regulation of G-protein coupled receptor protein signaling pathway; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process
Disease: Breast Cancer
Research Articles on PHB
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Product Notes
The Rat PHB phb (Catalog #AAA2532267) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2532267 CLIA Kit recognizes Rat PHB. It is sometimes possible for the material contained within the vial of "PHB (Prohibitin), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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