Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: Mouse muscle tissue Primary antibody: 1/500 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Rabbit anti-Human, Mouse NANOS1 Polyclonal Antibody | anti-NANOS1 antibody
NANOS1 Antibody
Gene Names
NANOS1; NOS1; NOS-1; SPGF12; EC_Rep1a
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
NANOS1; Polyclonal Antibody; NANOS1 Antibody; NOS1; SPGF12; anti-NANOS1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total NANOS1 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.9 mg/ml (varies by lot)
Sequence Length
292
Applicable Applications for anti-NANOS1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:10-1:50
Immunohistochemistry: 1:10-1:50
Immunogen
Synthetic peptide of human NANOS1
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: Mouse muscle tissue Primary antibody: 1/500 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: Mouse muscle tissue Primary antibody: 1/500 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Related Product Information for anti-NANOS1 antibody
Nanos1, also known as NOS1, is a 292 amino acid protein that localizes to the perinuclear region of the cytoplasm and contains one nanos-type zinc finger. Expressed at high levels in spermatogonia and present at lower levels in fetal ovaries, Nanos1 forms a complex with Pumilio 2 and functions to regulate the translation of select mRNAs, specifically via association with the 3¡¯-UTR of its mRNA targets. Additionally, Nanos1 is required for the establishment and maintenance of germline stem cells, as it prevents their premature entry into oogenesis.
Product Categories/Family for anti-NANOS1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,230 Da
NCBI Official Full Name
nanos homolog 1
NCBI Official Synonym Full Names
nanos homolog 1 (Drosophila)
NCBI Official Symbol
NANOS1
NCBI Official Synonym Symbols
NOS1; NOS-1; SPGF12; EC_Rep1a
NCBI Protein Information
nanos homolog 1
UniProt Protein Name
Nanos homolog 1
Protein Family
UniProt Gene Name
NANOS1
UniProt Synonym Gene Names
NOS1; NOS-1
UniProt Entry Name
NANO1_HUMAN
Uniprot Description
NANOS1: May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion. Belongs to the nanos family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 10q26.11
Cellular Component: cytoplasm; perinuclear region of cytoplasm
Molecular Function: protein binding; translation repressor activity
Biological Process: cell migration; negative regulation of translation
Disease: Spermatogenic Failure 12
Similar Products
Product Notes
The NANOS1 nanos1 (Catalog #AAA9415567) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NANOS1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NANOS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the NANOS1 nanos1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NANOS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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