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Western Blot (WB)
(Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human Nanos Homologue 1 Polyclonal Antibody
Nanos Homologue 1, CT (NANOS1, NOS1, Nanos homolog 1, EC_Rep1a)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
PurifiedPurified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
Nanos Homologue 1; Polyclonal Antibody; CT (NANOS1; NOS1; Nanos homolog 1; EC_Rep1a); Anti -Nanos Homologue 1; anti-Nanos Homologue 1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Nanos Homologue 1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunofluorescence: 1:10-50
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunofluorescence: 1:10-50
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunogen
NANOS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263~292 amino acids from the C-terminal region of human NANOS1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)
Immunofluorescence (IF)
(Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-Nanos Homologue 1 antibody
NANOS1 may regulate translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets.
Product Categories/Family for anti-Nanos Homologue 1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
nanos
Similar Products
Product Notes
The Nanos Homologue 1 (Catalog #AAA6012371) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nanos Homologue 1, CT (NANOS1, NOS1, Nanos homolog 1, EC_Rep1a) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nanos Homologue 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:50-100 Immunofluorescence: 1:10-50 Immunohistochemistry: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the Nanos Homologue 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nanos Homologue 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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