Rabbit anti-Human Nanos Homologue 1 Polyclonal Antibody | anti-NOS1 antibody

Nanos Homologue 1, CT (NANOS1, NOS1, Nanos homolog 1, EC_Rep1a) (Biotin)

Cat. Num. AAA6323706

• Gene Names: NANOS1; NOS1; SPGF12
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: Nanos Homologue 1; Polyclonal Antibody; CT (NANOS1; NOS1; Nanos homolog 1; EC_Rep1a) (Biotin); NANOS1; EC_Rep1a; anti-NOS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NOS1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IF: 1:10-50; IHC: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: NANOS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263~292 amino acids from the C-terminal region of human NANOS1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NOS1 antibody

NANOS1 may regulate translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets.

Product Categories/Family for anti-NOS1 antibody

Antibodies; DNA/RNA Translation

References

Strumane, K., Cancer Res. 66 (20), 10007-10015 (2006). Kurokawa, H., Dev. Growth Differ. 48 (3), 209-221 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 41688589
• NCBI GeneID: 340719
• UniProt Accession #: Q8WY41
• Molecular Weight: 30,230 Da
• NCBI Official Full Name: Nanos homolog 1
• NCBI Official Synonym Full Names: nanos homolog 1 (Drosophila)
• NCBI Official Symbol: NANOS1
• NCBI Official Synonym Symbols: NOS1; SPGF12
• NCBI Protein Information: nanos homolog 1; NOS-1; EC_Rep1a
• UniProt Protein Name: Nanos homolog 1
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NANOS1
• UniProt Synonym Gene Names: NOS1; NOS-1
• UniProt Entry Name: NANO1_HUMAN

NCBI Description

This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015]

Uniprot Description

NANOS1: May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion. Belongs to the nanos family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein binding; translation repressor activity; zinc ion binding; RNA binding

Biological Process: cell migration; negative regulation of translation

Disease: Spermatogenic Failure 12