Rabbit anti-Human, Mouse NALP12 Polyclonal Antibody | anti-NALP12 antibody

NALP12 (NACHT LRR and PYD Domains-containing Protein 12, CLR19.3, FCAS2, Monarch 1, Monarch-1, NLR Family Pyrin Domain Containing 12, NLRP12, PAN6, PYRIN Containing APAF1-like Protein 7, PYPAF7, Regulated by Nitric Oxide, RNO, RNO2) (Azide free) (HRP)

Cat. Num. AAA6489322

• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: NALP12; Polyclonal Antibody; NALP12 (NACHT LRR and PYD Domains-containing Protein 12; CLR19.3; FCAS2; Monarch 1; Monarch-1; NLR Family Pyrin Domain Containing 12; NLRP12; PAN6; PYRIN Containing APAF1-like Protein 7; PYPAF7; Regulated by Nitric Oxide; RNO; RNO2) (Azide free) (HRP); anti-NALP12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NLRP12. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NALP12 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NLRP12 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NLRP12 Antibody in HL-60 cell line lysates (35ug/lane). NLRP12 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of NLRP12 Antibody in mouse testis tissue lysates (35ug/lane). NLRP12 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-NALP12 antibody

Antibodies; Inflammasomes

References

1. Ye, Z., Mol. Cell. Biol. 28(5), 1841-1850 (2008). 2. Jeru, I., Proc. Natl. Acad. Sci. USA 105(5), 1614-1619 (2008). 3. Arthur, J.C., J. Immunol. 179(9), 6291-6296 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 15193292
• UniProt Accession #: P59046
• Molecular Weight: 120.173
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 12
• UniProt Gene Name: NLRP12
• UniProt Synonym Gene Names: NALP12; PYPAF7; RNO
• UniProt Entry Name: NAL12_HUMAN

Uniprot Description

NLRP12: May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B via IKK. Defects in NLRP12 are the cause of familial cold autoinflammatory syndrome type 2 (FCAS2). FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; caspase activator activity; ATP binding

Biological Process: release of cytoplasmic sequestered NF-kappaB; caspase activation; negative regulation of cytokine secretion; negative regulation of interleukin-6 biosynthetic process; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta secretion; signal transduction; regulation of caspase activity; negative regulation of signal transduction; negative regulation of Toll signaling pathway; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; negative regulation of protein amino acid autophosphorylation; positive regulation of MHC class I biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 secretion; regulation of interleukin-18 biosynthetic process; positive regulation of inflammatory response

Disease: Familial Cold Autoinflammatory Syndrome 2