Typical Testing Data
Human Protein Disulfide Isomerase ELISA Kit | PDI elisa kit
Human Protein Disulfide Isomerase ELISA Kit
Reactivity
Human
Synonyms
Protein Disulfide Isomerase; Human Protein Disulfide Isomerase ELISA Kit; PDI/DSI/ERBA2L/GIT/PDIA1/PO4DB/PO4HB/PROHB/Thbp/Cellular thyroid hormone-binding protein/collagen prolyl 4-hydroxylase beta/DSI/ERBA2L/GIT/glutathione-insulin transhydrogenase/P4Hbeta/p55/PDIA1procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); betapolypeptide (protein disulfide isomerase-associated 1)/PHDB/procollagen-proline; betapolypeptide/PROHBprocollagen-proline; betapolypeptide (protein disulfide isomerase/thyroid hormone binding protein p55)/Prolyl 4-hydroxylase subunit beta/prolyl 4-hydroxylase; beta polypeptide/protein disulfide isomerase family A; member 1/protein disulfide isomerase/oxidoreductase/protein disulfide isomerase-associated 1/protein disulfide-isomerase/protocollagen hydroxylase/thyroid hormone-binding protein p55; PDI elisa kit
Reactivity
Human
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
Typical Testing Data
Typical Testing Data
Typical Standard Curve
Typical Standard Curve
Related Product Information for PDI elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PDI antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PDI antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PDI amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PDI can be calculated.
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Product Notes
The Human PDI (Catalog #AAA764478) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA764478 ELISA Kit recognizes Human PDI. It is sometimes possible for the material contained within the vial of "Protein Disulfide Isomerase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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