Rabbit anti-Human CSHL1 Polyclonal Antibody | anti-CSHL1 antibody

CSHL1, CT (CSHL1, CSHP1, CSL, Chorionic somatomammotropin hormone-like 1, Lactogen-like) (APC)

Cat. Num. AAA6289287

• Gene Names: CSHL1; CSL; CS-5; CSHP1; hCS-L
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CSHL1; Polyclonal Antibody; CT (CSHL1; CSHP1; CSL; Chorionic somatomammotropin hormone-like 1; Lactogen-like) (APC); Lactogen-like; anti-CSHL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CSHL1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CSHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-152 amino acids from the C-terminal region of human CSHL1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CSHL1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CSHL1 antibody detected the CSHL1 protein (arrow).)

Immunohistochemistry (IHC)

(CSHL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CSHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CSHL1 antibody

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. This particular family member is expressed in placental villi, although it was originally thought to be a pseudogene. In fact, alternative splicing suggests that the majority of the transcripts would be unable to express a secreted protein. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-CSHL1 antibody

Antibodies; Hormones; Steroids

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Misra-Press, A., et al. J. Biol. Chem. 269(37):23220-23229(1994) MacLeod, J.N., et al. J. Biol. Chem. 267(20):14219-14226(1992) Vnencak-Jones, C.L., et al. Science 250(4988):1745-1748(1990).

NCBI and Uniprot Product Information

• NCBI GI #: 12545381
• NCBI GeneID: 1444
• NCBI Accession #: NP_072103.1
• NCBI GenBank Nucleotide #: NM_022581.1
• UniProt Accession #: Q14406; Q0VDB2; D3DU26; D3DU27
• Molecular Weight: 25,391 Da
• NCBI Official Full Name: chorionic somatomammotropin hormone-like 1 isoform 2
• NCBI Official Synonym Full Names: chorionic somatomammotropin hormone-like 1
• NCBI Official Symbol: CSHL1
• NCBI Official Synonym Symbols: CSL; CS-5; CSHP1; hCS-L
• NCBI Protein Information: chorionic somatomammotropin hormone-like 1; growth hormone cluster; chorionic somatomammotropin CS-5
• UniProt Protein Name: Chorionic somatomammotropin hormone-like 1
• Protein Family: Chorionic somatomammotropin hormone
• UniProt Gene Name: CSHL1
• UniProt Synonym Gene Names: CSHP1; CSL; Chorionic somatomammotropin-like
• UniProt Entry Name: CSHL_HUMAN

NCBI Description

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. This particular family member is expressed in placental villi, although it was originally thought to be a pseudogene. In fact, alternative splicing suggests that the majority of the transcripts would be unable to express a secreted protein. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

CSHL1: May be a novel gestational hormone required to compensate for absence of other members of the GH/CS cluster during gestation. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular region

Molecular Function: hormone activity; metal ion binding