Human Klotho (KL) ELISA Kit | KL elisa kit

Human Klotho (KL) ELISA Kit

Cat. Num. AAA2601069

• Reactivity: Human
• Synonyms: Klotho (KL); Human Klotho (KL) ELISA Kit; KL elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 1012

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: Up to 0.05 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KL elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human KL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 2618596
• NCBI GeneID: 9365
• Molecular Weight: 62,135 Da
• NCBI Official Full Name: klotho
• NCBI Official Synonym Full Names: klotho
• NCBI Official Symbol: KL
• NCBI Protein Information: klotho
• UniProt Protein Name: Klotho
• Protein Family: Klotho
• UniProt Gene Name: KL
• UniProt Entry Name: KLOT_HUMAN

NCBI Description

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]

Uniprot Description

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Hydrolase; EC 3.2.1.31

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: extracellular space; integral to plasma membrane; integral to membrane; plasma membrane; extracellular region

Molecular Function: vitamin D binding; signal transducer activity; beta-glucuronidase activity; fibroblast growth factor binding; beta-glucosidase activity; hormone activity; fibroblast growth factor receptor binding

Biological Process: calcium ion homeostasis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; carbohydrate metabolic process; energy reserve metabolic process; insulin receptor signaling pathway; innate immune response; positive regulation of bone mineralization; acute inflammatory response; aging

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial