Rabbit anti-Human NADH Dehydrogenase Fe-S Protein 4 Polyclonal Antibody | anti-NDUFS4 antibody

NADH Dehydrogenase Fe-S Protein 4, CT (NADH Dehydrogenase [Ubiquinone] Iron-sulfur Protein 4 Mitochondrial, NDUFS4, AQDQ, Complex I-18kD, CI-18kD, Complex I-AQDQ, CI-AQDQ, NADH-ubiquinone Oxidoreductase 18kD Subunit) (PE)

Cat. Num. AAA6489272

• Gene Names: NDUFS4; AQDQ; CI-18
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: NADH Dehydrogenase Fe-S Protein 4; Polyclonal Antibody; CT (NADH Dehydrogenase [Ubiquinone] Iron-sulfur Protein 4 Mitochondrial; NDUFS4; AQDQ; Complex I-18kD; CI-18kD; Complex I-AQDQ; CI-AQDQ; NADH-ubiquinone Oxidoreductase 18kD Subunit) (PE); NADH Dehydrogenase (Ubiquinone) Fe-S Protein 4; 18kDa (NADH-coenzyme Q Reductase); anti-NDUFS4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NDUFS4.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NDUFS4 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NDUFS4 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NDUFS4 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). NDUFS4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with NDUFS4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of ATDC5 cells using SLC22A23 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of NDUFS4 (arrow) using rabbit polyclonal NDUFS4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDUFS4 gene.)

Product Categories/Family for anti-NDUFS4 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Panelli, D., et.al., Biochimie 90(10), 1452-1460 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4505369
• NCBI GeneID: 4724
• NCBI Accession #: NP_002486.1
• NCBI GenBank Nucleotide #: NM_002495.2
• UniProt Accession #: O43181; Q9BS69
• Molecular Weight: 20,108 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
• NCBI Official Symbol: NDUFS4
• NCBI Official Synonym Symbols: AQDQ; CI-18
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; CI-18 kDa; CI-AQDQ; NADH dehydrogenase (ubiquinone) iron-sulfur protein 4; NADH-coenzyme Q reductase, 18-KD; NADH-u
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
• Protein Family: NADH dehydrogenase [ubiquinone] iron-sulfur protein
• UniProt Gene Name: NDUFS4
• UniProt Synonym Gene Names: CI-18 kDa; CI-AQDQ
• UniProt Entry Name: NDUS4_HUMAN

NCBI Description

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes. [provided by RefSeq, Jul 2008]

Uniprot Description

NDUFS4: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I NDUFS4 subunit family.

Protein type: EC 1.6.99.3; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.6.5.3

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial respiratory chain complex I assembly; positive regulation of fibroblast proliferation; cAMP-mediated signaling; regulation of protein amino acid phosphorylation; response to cAMP; mitochondrial electron transport, NADH to ubiquinone; cellular respiration; brain development

Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency