Rabbit anti-Human NADH Dehydrogenase Fe-S Protein 4 Polyclonal Antibody | anti-NDUFS4 antibody

NADH Dehydrogenase Fe-S Protein 4, CT (NADH Dehydrogenase [Ubiquinone] Iron-sulfur Protein 4 Mitochondrial, NDUFS4, AQDQ, Complex I-18kD, CI-18kD, Complex I-AQDQ, CI-AQDQ, NADH-ubiquinone Oxidoreductase 18kD Subunit) (Azide free) (HRP)

Cat. Num. AAA6489266

• Gene Names: NDUFS4; AQDQ; CI-18
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: NADH Dehydrogenase Fe-S Protein 4; Polyclonal Antibody; CT (NADH Dehydrogenase [Ubiquinone] Iron-sulfur Protein 4 Mitochondrial; NDUFS4; AQDQ; Complex I-18kD; CI-18kD; Complex I-AQDQ; CI-AQDQ; NADH-ubiquinone Oxidoreductase 18kD Subunit) (Azide free) (HRP); NADH Dehydrogenase (Ubiquinone) Fe-S Protein 4; 18kDa (NADH-coenzyme Q Reductase); anti-NDUFS4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NDUFS4.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NDUFS4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NDUFS4 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NDUFS4 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). NDUFS4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with NDUFS4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of ATDC5 cells using SLC22A23 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of NDUFS4 (arrow) using rabbit polyclonal NDUFS4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDUFS4 gene.)

Product Categories/Family for anti-NDUFS4 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Panelli, D., et.al., Biochimie 90(10), 1452-1460 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4505369
• NCBI GeneID: 4724
• NCBI Accession #: NP_002486.1
• NCBI GenBank Nucleotide #: NM_002495.2
• UniProt Accession #: O43181; Q9BS69
• Molecular Weight: 20,108 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
• NCBI Official Symbol: NDUFS4
• NCBI Official Synonym Symbols: AQDQ; CI-18
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; CI-18 kDa; CI-AQDQ; NADH dehydrogenase (ubiquinone) iron-sulfur protein 4; NADH-coenzyme Q reductase, 18-KD; NADH-u
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
• Protein Family: NADH dehydrogenase [ubiquinone] iron-sulfur protein
• UniProt Gene Name: NDUFS4
• UniProt Synonym Gene Names: CI-18 kDa; CI-AQDQ
• UniProt Entry Name: NDUS4_HUMAN

NCBI Description

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes. [provided by RefSeq, Jul 2008]

Uniprot Description

NDUFS4: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I NDUFS4 subunit family.

Protein type: EC 1.6.99.3; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.6.5.3

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial respiratory chain complex I assembly; positive regulation of fibroblast proliferation; cAMP-mediated signaling; regulation of protein amino acid phosphorylation; response to cAMP; mitochondrial electron transport, NADH to ubiquinone; cellular respiration; brain development

Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency