Western Blot (WB)
(Western blot detection of N-WASP in 3T3 cell lysates using N-WASP Rabbit pAb(1:1000 diluted). Predicted band size:55kDa. Observed band size:65kDa.)
Rabbit anti-Human, Mouse N WASP Polyclonal Antibody | anti-WASL antibody
N WASP Rabbit pAb
Gene Names
WASL; NWASP; N-WASP
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity purified
Synonyms
N WASP; Polyclonal Antibody; N WASP Rabbit pAb; Neural Wiskott-Aldrich syndrome protein; NWASP; WASPB; N-WASP; anti-WASL antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-WASL antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:20-1:50
ICC/IF: 1:50
FC/FACS: 1:20-1:100
IHC: 1:20-1:50
ICC/IF: 1:50
FC/FACS: 1:20-1:100
Immunogen
A synthetic peptide of human N-WASP
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cytoplasm, Cytoskeleton, Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot detection of N-WASP in 3T3 cell lysates using N-WASP Rabbit pAb(1:1000 diluted). Predicted band size:55kDa. Observed band size:65kDa.)
Western Blot (WB)
(Western blot detection of N-WASP in 3T3 cell lysates using N-WASP Rabbit pAb(1:1000 diluted). Predicted band size:55kDa. Observed band size:65kDa.)
Related Product Information for anti-WASL antibody
This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:9422512, PubMed:16767080, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828). Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization (PubMed:9422512, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828). Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia (PubMed:9422512). In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (PubMed:16767080). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis.
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:9422512, PubMed:16767080, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828). Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization (PubMed:9422512, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828). Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia (PubMed:9422512). In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (PubMed:16767080). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
505
NCBI Official Full Name
neural Wiskott-Aldrich syndrome protein
NCBI Official Synonym Full Names
Wiskott-Aldrich syndrome-like
NCBI Official Symbol
WASL
NCBI Official Synonym Symbols
NWASP; N-WASP
NCBI Protein Information
neural Wiskott-Aldrich syndrome protein
UniProt Protein Name
Neural Wiskott-Aldrich syndrome protein
Protein Family
UniProt Gene Name
WASL
UniProt Synonym Gene Names
N-WASP
UniProt Entry Name
WASL_HUMAN
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Product Notes
The WASL wasl (Catalog #AAA4752138) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The N WASP Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's N WASP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:20-1:50 ICC/IF: 1:50 FC/FACS: 1:20-1:100. Researchers should empirically determine the suitability of the WASL wasl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N WASP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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