Western Blot (WB)
(Western blot analysis of lysates from ZF4 cell line, Zebrafish, zebra fish muscle tissue lysate(from left to right), using HIST1H2BJ Antibody (Center). MBS9209216 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit HIST1H2B Polyclonal Antibody | anti-HIST1H2BJ antibody
HIST1H2B Antibody (Center)
Western Blot (WB)
(Western blot analysis of lysates from ZF4 cell line, Zebrafish, zebra fish muscle tissue lysate(from left to right), using HIST1H2BJ Antibody (Center). MBS9209216 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from ZF4 cell line, Zebrafish, zebra fish muscle tissue lysate(from left to right), using HIST1H2BJ Antibody (Center). MBS9209216 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Immunohistochemistry (IHC)
(HIST1H2B antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2B antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(HIST1H2B antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2B antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(HIST1H2B Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(HIST1H2B Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(HIST1H2B Antibody (Center) western blot analysis in CEM,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2B antibody detected the HIST1H2B protein (arrow).)
Western Blot (WB)
(HIST1H2B Antibody (Center) western blot analysis in CEM,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2B antibody detected the HIST1H2B protein (arrow).)
for the nucleosome structure of the chromosomal fiber in
eukaryotes. Two molecules of each of the four core histones (H2A,
H2B, H3, and H4) form an octamer, around which approximately 146 bp
of DNA is wrapped in repeating units, called nucleosomes. The
linker histone, H1, interacts with linker DNA between nucleosomes
and functions in the compaction of chromatin into higher order
structures. This gene is intronless and encodes a member of the
histone H2B family. Transcripts from this gene lack polyA tails but
instead contain a palindromic termination element. This gene is
found in the histone microcluster on chromosome 6p21.33. [provided
by RefSeq].
Benyamin, B., et al. Am. J. Hum. Genet. 84(1):60-65(2009)
Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
Beck, H.C., et al. Mol. Cell Proteomics 5(7):1314-1325(2006)
Pavri, R., et al. Cell 125(4):703-717(2006)
NCBI and Uniprot Product Information
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Jul 2008]
Uniprot Description
HIST1H2BJ: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Belongs to the histone H2B family.
Chromosomal Location of Human Ortholog: 6p22.1
Cellular Component: nucleoplasm; extracellular space; nucleosome; nucleus
Molecular Function: DNA binding; protein heterodimerization activity
Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture; defense response to Gram-positive bacterium; innate immune response in mucosa; antibacterial humoral response
Research Articles on HIST1H2BJ
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Product Notes
The HIST1H2BJ hist1h2bj (Catalog #AAA9209216) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 57-86. The HIST1H2B Antibody (Center) reacts with Human (Predicted Reactivity: Xenopus, Rat, Mouse, Bovine, Monkey, Chicken, Zebrafish) and may cross-react with other species as described in the data sheet. AAA Biotech's HIST1H2B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the HIST1H2BJ hist1h2bj for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIST1H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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