Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse N-Acetylgalactosamine-6-Sulfatase Polyclonal Antibody | anti-GAS antibody
Polyclonal Antibody to N-Acetylgalactosamine-6-Sulfatase (GAS)
Gene Names
Galns; mFLJ00319
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
N-Acetylgalactosamine-6-Sulfatase; Polyclonal Antibody; Polyclonal Antibody to N-Acetylgalactosamine-6-Sulfatase (GAS); anti-GAS antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GAS. It has beenselected for its ability to recognize GAS in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DNK AKPNIPVYRD WEMVGRFYEE FPINRKTGEA NLTQLYTQEA LDFIQTQHAR QSPFFLYWAI DATHAPVYAS RQFLGTSLRG RYGDAVREID DSVGKILSLL QNLGISKNTF VFFTSDNGAA LISAPNEGGS NGPFLCGKQT TFEGGMREPA IAWWPGHIAA GQVSHQLGSI MDLFTTSLSL AGLKPPSDRV
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DNK AKPNIPVYRD WEMVGRFYEE FPINRKTGEA NLTQLYTQEA LDFIQTQHAR QSPFFLYWAI DATHAPVYAS RQFLGTSLRG RYGDAVREID DSVGKILSLL QNLGISKNTF VFFTSDNGAA LISAPNEGGS NGPFLCGKQT TFEGGMREPA IAWWPGHIAA GQVSHQLGSI MDLFTTSLSL AGLKPPSDRV
Applicable Applications for anti-GAS antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
GAS (Asp168~Asp377)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant GAS (Asp168~Asp377) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040143
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,673 Da
NCBI Official Full Name
N-acetylgalactosamine-6-sulfatase isoform 2
NCBI Official Synonym Full Names
galactosamine (N-acetyl)-6-sulfate sulfatase
NCBI Official Symbol
Galns
NCBI Official Synonym Symbols
mFLJ00319
NCBI Protein Information
N-acetylgalactosamine-6-sulfatase
UniProt Protein Name
N-acetylgalactosamine-6-sulfatase
Protein Family
UniProt Gene Name
Galns
UniProt Synonym Gene Names
Chondroitinase; GalNAc6S sulfatase
Uniprot Description
GALNS: Defects in GALNS are the cause of mucopolysaccharidosis type 4A (MPS4A); also known as Morquio A syndrome. MPS4A is a form of mucopolysaccharidosis type 4, an autosomal recessive lysosomal storage disease characterized by intracellular accumulation of keratan sulfate and chondroitin-6-sulfate. Key clinical features include short stature, skeletal dysplasia, dental anomalies, and corneal clouding. Intelligence is normal and there is no direct central nervous system involvement, although the skeletal changes may result in neurologic complications. There is variable severity, but patients with the severe phenotype usually do not survive past the second or third decade of life. Belongs to the sulfatase family.
Protein type: EC 3.1.6.4; Glycan Metabolism - glycosaminoglycan degradation; Hydrolase
Chromosomal Location of Human Ortholog: 8|8 E1
Cellular Component: lysosome
Molecular Function: catalytic activity; hydrolase activity; metal ion binding; N-acetylgalactosamine-6-sulfatase activity; sulfuric ester hydrolase activity
Biological Process: metabolic process
Similar Products
Product Notes
The GAS galns (Catalog #AAA2028212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to N-Acetylgalactosamine-6-Sulfatase (GAS) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's N-Acetylgalactosamine-6-Sulfatase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the GAS galns for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DNK AKPNIPVYRD WEMVGRFYEE FPINRKTGEA NLTQLYTQEA LDFIQTQHAR QSPFFLYWAI DATHAPVYAS RQFLGTSLRG RYGDAVREID DSVGKILSLL QNLGISKNTF VFFTSDNGAA LISAPNEGGS NGPFLCGKQT TFEGGMREPA IAWWPGHIAA GQVSHQLGSI MDLFTTSLSL AGLKPPSDRV. It is sometimes possible for the material contained within the vial of "N-Acetylgalactosamine-6-Sulfatase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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