Goat MYRIP/SLAC2C Polyclonal Antibody | anti-MYRIP antibody

Goat anti-MYRIP/SLAC2C Antibody

Cat. Num. AAA420431

• Gene Names: MYRIP; SLAC2C; SLAC2-C
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: MYRIP/SLAC2C; Polyclonal Antibody; Goat anti-MYRIP/SLAC2C Antibody; MYRIP; SLAC2C; myosin VIIA and Rab interacting protein; MyRIP; SLAC2-C; exophilin; exophilin 8; DKFZp586F1018; rab effector MYRIP; Slp homologue lacking C2 domains; synaptotagmin-like protein homologue lacking C2 domains-c; FLJ44025; MGC130034; MGC130035; MYRIP antibody; SLAC2C antibody; myosin VIIA and Rab interacting protein antibody; MyRIP antibody; SLAC2-C antibody; exophilin antibody; exophilin 8 antibody; DKFZp586F1018 antibody; rab effector MYRIP antibody; Slp homologue lacking C2 domains antibody; synaptotagmin-like protein homologue lacking C2 domains-c antibody; FLJ44025 antibody; MGC130034 antibody; MGC130035 antibody; OTTHUMP00000209206 antibody; MYRIP / SLAC2C; anti-MYRIP antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KDLMEPALESAVMY
• Sequence Length: 859

• Applicable Applications for anti-MYRIP antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Western Blot: Approx 90kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 95.7kDa according to NP_056275.2). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-KDLMEPALESAVMY, from the C Terminus of the protein sequence according to NP_056275.2.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 59853352
• NCBI GeneID: 25924
• NCBI Accession #: NP_056275.2
• NCBI GenBank Nucleotide #: NM_015460.3
• Molecular Weight: 83,948 Da
• NCBI Official Full Name: rab effector MyRIP isoform a
• NCBI Official Synonym Full Names: myosin VIIA and Rab interacting protein
• NCBI Official Symbol: MYRIP
• NCBI Official Synonym Symbols: SLAC2C; SLAC2-C
• NCBI Protein Information: rab effector MyRIP
• UniProt Protein Name: Rab effector MyRIP
• Protein Family: Rab effector
• UniProt Gene Name: MYRIP
• UniProt Synonym Gene Names: SLAC2C; SlaC2-c; Slp homolog lacking C2 domains c
• UniProt Entry Name: MYRIP_HUMAN

Uniprot Description

MYRIP: Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments. Functions as a protein kinase A-anchoring protein (AKAP). May act as a scaffolding protein that links PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: actin cytoskeleton; apical plasma membrane; exocyst; melanosome; perinuclear region of cytoplasm; photoreceptor outer segment; secretory granule membrane; synapse; transport vesicle

Molecular Function: actin binding; myosin binding; protein binding; protein kinase A binding; Rab GTPase binding; zinc ion binding

Biological Process: intracellular protein transport; positive regulation of insulin secretion